Validation and Optimization of a Stable Isotope-Labeled Substrate Assay for Measuring AGAT Activity.

Alex Lee, Lucas Anderson, Ilona Tkachyova, Michael B Tropak, Dahai Wang, Andreas Schulze

Abstract readValidation Study

In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Alex LeeDepartment of Biochemistry, University of Toronto, Toronto, ON M5S 1A1, Canada.

Lucas AndersonGenetics and Genome Biology, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.

Ilona TkachyovaGenetics and Genome Biology, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.

Michael B TropakGenetics and Genome Biology, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.

Dahai WangGenetics and Genome Biology, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.

Andreas SchulzeDepartment of Biochemistry, University of Toronto, Toronto, ON M5S 1A1, Canada.ORCID 0000-0001-8491-1095

Funding

Association for Creatine Disorders n/a

6 · The paper itself

Abstract

L-arginine: glycine amidinotransferase (AGAT) gained academic interest as the rate-limiting enzyme in creatine biosynthesis and its role in the regulation of creatine homeostasis. Of clinical relevance is the diagnosis of patients with AGAT deficiency but also the potential role of AGAT as therapeutic target for the treatment of another creatine deficiency syndrome, guanidinoacetate N-methyltransferase (GAMT) deficiency. Applying a stable isotope-labeled substrate method, we utilized ARG 15N

Indexed as

AmidinotransferasesIsotope LabelingAnimalsArginineBrainEnzyme AssaysGlycineGuanidinoacetate N-MethyltransferaseHumansKidneyLiverMaleMiceMice, Inbred C57BLSubstrate SpecificityAmidinotransferasesArginineGlycineglycine amidinotransferaseGuanidinoacetate N-MethyltransferaseAGAT activitycreatine deficiency syndromesenzyme assayisotope-labeled

Identifiers

PMID39684202

DOI10.3390/ijms252312490

PMCPMC11641458

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.