Evidence map›Paper›PMID 39683737›Full record

ArticleMolecules (Basel, Switzerland)2024

Expression, Purification, and Characterisation of Recombinant Alginate Lyase (

Coleen E Grobler, Blessing Mabate, Alaric Prins, Marilize Le Roes-Hill, Brett I Pletschke

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Coleen E GroblerEnzyme Science Programme (ESP), Department of Biochemistry, Microbiology and Bioinformatics, Rhodes University, Makhanda 6140, South Africa.
Blessing MabateEnzyme Science Programme (ESP), Department of Biochemistry, Microbiology and Bioinformatics, Rhodes University, Makhanda 6140, South Africa.ORCID 0000-0002-3584-5703
Alaric PrinsApplied Microbial and Health Biotechnology Institute, Cape Peninsula University of Technology, Bellville 7535, South Africa.ORCID 0000-0002-1797-3648
Marilize Le Roes-HillApplied Microbial and Health Biotechnology Institute, Cape Peninsula University of Technology, Bellville 7535, South Africa.ORCID 0000-0002-1930-2637
Brett I PletschkeEnzyme Science Programme (ESP), Department of Biochemistry, Microbiology and Bioinformatics, Rhodes University, Makhanda 6140, South Africa.ORCID 0000-0001-8117-1730

Funding

Department of Science and Innovation Node of the Industrial Biocatalysis HubNational Research Foundation 138015Rhodes University Henderson Postgraduate Scholarship
6 · The paper itself

Abstract

Alginate, a polysaccharide found in brown seaweeds, has regularly gained attention for its potential use as a source of bioactive compounds. However, it is structurally complex with a high molecular weight, limiting its application. Alginate oligosaccharides (AOS) are small, soluble fragments, making them more bioavailable. Alginate hydrolysis by enzymes is the preferred method for AOS production. Commercially available alginate lyases are limited, expensive, and sometimes exhibit unsatisfactory activity, making the search for novel alginate lyases with improved activity indispensable. The aims of this study were to codon-optimise, synthesise, express, purify, and characterise a recombinant alginate lyase, AL2, from

Indexed as

AlginatesOligosaccharidesPolysaccharide-LyasesRecombinant ProteinsEnzyme StabilityEscherichia coliGene ExpressionHydrogen-Ion ConcentrationTemperatureAlginatesOligosaccharidespoly(beta-D-mannuronate) lyasePolysaccharide-LyasesRecombinant Proteinsalginatealginate lyasealginate oligosaccharidesbrown seaweedsenzymatic hydrolysis

Identifiers

PMID39683737
PMCPMC11643991

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.