Evidence map›Paper›PMID 39683071›Full record

ArticlePlants (Basel, Switzerland)2024

Development of a Tandem Mass Spectral Library for the Detection of Triterpenoids in Plant Metabolome Based on Reference Standards.

Bibi Zareena, Syed Usama Y Jeelani, Adeeba Khadim, Arslan Ali, Jalal Uddin, Satyajit D Sarker, Matthias Rainer, Shaden A M Khalifa, Hesham R El-Seedi, Muhammad Ramzan and 1 more

Abstract read
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In one paragraph

Article in Plants (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Bibi ZareenaH.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
Syed Usama Y JeelaniH.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.ORCID 0000-0002-6883-8489
Adeeba KhadimH.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
Arslan AliDr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.ORCID 0000-0001-5754-6483
Jalal UddinDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Khalid University, Abha 61421, Saudi Arabia.ORCID 0000-0002-8613-4493
Satyajit D SarkerSchool of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, James Parsons Building Byrom Street, Liverpool L3 3AF, UK.ORCID 0000-0003-4038-0514
Matthias RainerInstitute for Analytical Chemistry and Radiochemistry, University of Innsbruck, A-6020 Innsbruck, Austria.ORCID 0000-0001-9215-8515
Shaden A M KhalifaNeurology and Psychiatry Department, Capio Saint Göran's Hospital, Sankt Göransplan 1, 112 19 Stockholm, Sweden.
Hesham R El-SeediDepartment of Chemistry, Faculty of Science, Islamic University of Madinah, Madinah 42351, Saudi Arabia.ORCID 0000-0002-2519-6690
Muhammad RamzanH.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
Syed Ghulam MusharrafH.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.ORCID 0000-0003-2026-3302

Funding

Ignite - National Technology Fund ICTRDF/TR&D/2014/26
6 · The paper itself

Abstract

Plant triterpenoids represent a diverse group of secondary metabolites and are thought to be valuable for therapeutic applications. For drug development, lead optimization, better knowledge of biological pathways, and high-throughput detection of secondary metabolites in plant extracts are crucial. This paper describes a qualitative method for the rapid and accurate identification of various triterpenoids in plant extracts using the LC-HR-ESI-MS/MS tool in combination with the data-dependent acquisition (DD) approach. A total of 44 isolated, purified, and characterized triterpenoids were analyzed. HR-MS spectra and tandem mass spectra (MS/MS) of each compound were recorded in the positive ionization mode in two different sets of collisional energies, i.e., (25-62.5 eV), and fixed collisional energies (10, 20, 30, and 40 eV). As a result, three triterpenoids were identified in all plant extracts using the retention time, high-resolution mass spectra, and/or MS/MS spectra. The developed method will be helpful with other plant extracts/botanicals, as well as in the search for new triterpenoids in the kingdom Plantae.

Indexed as

electrospray ionization–mass spectrometryESI-QTOF-MS/MS methodgeneration of spectral librarytriterpenoids

Identifiers

PMID39683071
PMCPMC11644824

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.