Evidence map›Paper›PMID 39683069›Full record

ArticlePlants (Basel, Switzerland)2024

Development and Validation of One-Step Reverse Transcription-Droplet Digital PCR for Plum Pox Virus Detection and Quantification from Plant Purified RNA and Crude Extract.

Giorgia Bertinelli, Lorenza Tizzani, Marta Luigi, Simona Monticelli, Vincenza Ilardi

Abstract read
In one paragraph

Article in Plants (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Foods (Basel, Switzerland) · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Giorgia BertinelliCREA Research Centre for Plant Protection and Certification, Via C.G. Bertero 22, 00156 Rome, Italy.ORCID 0000-0003-0016-9239
Lorenza TizzaniCREA Research Centre for Plant Protection and Certification, Via C.G. Bertero 22, 00156 Rome, Italy.ORCID 0000-0003-0609-9876
Marta LuigiCREA Research Centre for Plant Protection and Certification, Via C.G. Bertero 22, 00156 Rome, Italy.ORCID 0000-0002-4186-4687
Simona MonticelliCREA Research Centre for Olive, Fruit and Citrus Crops, Via di Fioranello 52, 00134 Rome, Italy.ORCID 0000-0002-0048-7942
Vincenza IlardiCREA Research Centre for Plant Protection and Certification, Via C.G. Bertero 22, 00156 Rome, Italy.ORCID 0000-0003-3657-1158

Funding

Italian Ministry of Agriculture (MiPAAF) project BIOTECH-BIOSOSFRU
6 · The paper itself

Abstract

Plum pox virus (PPV) is the etiological agent of sharka, the most important viral disease of stone fruit worldwide. In this study, a one-step reverse transcription real-time PCR test (RT-qPCR) was modified and translated as a one-step RT-droplet digital PCR (RT-ddPCR) for sensitive, direct, and accurate detection and quantification of PPV. The modified RT-qPCR and RT-ddPCR PPV detection tests were validated using both plant purified total RNA (TRNA) and crude extract as templates. The proposed tests were sensitive, specific, selective, repeatable, and reproducible in detecting PPV from fresh, lyophilized, and

Indexed as

plum pox virusPPV strainsPrunus spp.real-time PCRRT-ddPCRsharka

Identifiers

PMID39683069
PMCPMC11644555

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.