Evidence map›Paper›PMID 39682800›Full record

ArticleFoods (Basel, Switzerland)2024

A Novel Normalized Quantitative Real-Time PCR Approach for Ensuring Roe Deer (

Bukola M Adenuga, Rita Biltes, Caterina Villa, Joana Costa, Anita Spychaj, Magdalena Montowska, Isabel Mafra

Abstract read
In one paragraph

Article in Foods (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Bukola M AdenugaLAQV/REQUIMTE, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.ORCID 0000-0002-9908-9185
Rita BiltesLAQV/REQUIMTE, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.ORCID 0000-0001-5410-4831
Caterina VillaLAQV/REQUIMTE, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.ORCID 0000-0002-9471-3612
Joana CostaLAQV/REQUIMTE, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.ORCID 0000-0002-8229-2902
Anita SpychajDepartment of Meat Technology, Poznań University of Life Sciences, ul. Wojska Polskiego 31, 60-624 Poznań, Poland.ORCID 0000-0003-3688-8315
Magdalena MontowskaDepartment of Meat Technology, Poznań University of Life Sciences, ul. Wojska Polskiego 31, 60-624 Poznań, Poland.ORCID 0000-0002-6331-5726
Isabel MafraLAQV/REQUIMTE, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.ORCID 0000-0001-5311-8895

Funding

Fundação para a Ciência e Tecnologia 2021.03583.CEECIND/CP1662/CT0012Fundação para a Ciência e Tecnologia 2021.03670.CEECIND/CP1662/CT0011Fundação para a Ciência e Tecnologia 2023.07644.CEECINDFundação para a Ciência e Tecnologia UIDB/50006/2020National Science Centre 2020/37/B/NZ9/00082
6 · The paper itself

Abstract

Roe deer meat is a prized game product in many European countries. However, concerns exist regarding the accuracy of the amount of declared roe deer in processed game meat foods. This study aimed to develop a reliable method for the detection and quantification of roe deer in commercialized game meat products. A TaqMan probe-based quantitative real-time PCR (qPCR) assay was designed, targeting a single-copy 120-bp region of the roe deer agouti signaling protein (ASIP) encoding gene. The method employed the normalized ∆Cq approach to establish a calibration curve for roe deer detection and quantification within 0.05-50% (

Indexed as

agouti signaling protein (ASIP) genefood authenticationgame meat productsnuclear markerqPCRroe deer

Identifiers

PMID39682800
PMCPMC11640146

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.