Evidence map›Paper›PMID 39682506›Full record

ArticleAnimals : an open access journal from MDPI2024

Prolactin Inhibition Promotes Follicle Recruitment by Increasing

Sicong Yue, Chunhui Duan, Yong Wang, Xiangyun Li, Ruochen Yang, Yu Li, Xiangyu Chen, Yueqin Liu, Yingjie Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Sicong YueCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China.
Chunhui DuanCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China.
Yong WangInstitute of Animal Nutrition and Feed, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China.ORCID 0000-0002-5263-1002
Xiangyun LiCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China.
Ruochen YangCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China.
Yu LiCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China.
Xiangyu ChenBaoding Livestock Husbandry Workstation, Baoding 071000, China.
Yueqin LiuCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China.
Yingjie ZhangCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China.

Funding

National Modern Agricultural Industry Technology System Construction Project of China CARS-38
6 · The paper itself

Abstract

Prolactin (PRL) plays a key role in the growth and ovulation of animal follicles, but its impact on follicular recruitment in ewes remains uncertain. In this study, a total of sixteen healthy ewes (Hu sheep, aged 2-3 years, with continuous reproduction and housed separately), matched for parity and weight (52.98 ± 0.96 kg), were randomly assigned to two groups: a control group (C) and a treatment group (T, PRL inhibition). Ovaries were collected in vivo after anesthesia during the estrus stage, and tissue morphology was observed using hematoxylin-eosin (HE) staining. By using RNA sequencing on the ovaries of C and T groups and conducting bioinformatics analysis, the essential genes and pathways involved in the regulation of PRL inhibition were pinpointed. Subcellular localization of key genes in ovarian tissue was determined using a fluorescence in situ hybridization (FISH) assay and immunohistochemistry. The function of key genes was validated using knockout and overexpression techniques. During the estrus phase, we noted a marked rise in the count of large follicles within ovarian tissue following the inhibition of prolactin. In total, 328 differentially expressed genes (DEGs) were detected, with 162 upregulated and 166 downregulated. The results indicated that inhibiting PRL primarily influences follicle recruitment by acting on the target gene

Indexed as

estrusewefollicle recruitmentPIKfyveprolactin

Identifiers

PMID39682506
PMCPMC11639777

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.