Evidence map›Paper›PMID 39682403›Full record

ArticleAnimals : an open access journal from MDPI2024

Development of a Rapid Visual Detection Assay for Duck Tembusu Virus Using RT-LAMP-CRISPR/Cas12a.

Jimin Chen, Dagang Tao, Fan Yang, Chengfu Pan, Xinguo Bao, Shengsong Xie, Ping Gong, Changzhi Zhao, Ruiyi Lin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jimin ChenCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Dagang TaoKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China.
Fan YangCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Chengfu PanCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Xinguo BaoCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Shengsong XieKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China.ORCID 0000-0002-8301-877X
Ping GongAnimal Husbandry and Veterinary Research Institute, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China.
Changzhi ZhaoKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China.
Ruiyi LinCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.ORCID 0000-0003-3255-5900

Funding

National Natural Science Foundation of China 31702109Natural Science Foundation of Fujian Province 2017J01596Special Fund for Science and Technology Innovation of Fujian Agriculture and Forestry University KFb22064XA
6 · The paper itself

Abstract

Duck Tembusu virus (DTMUV) is an emerging flavivirus that has inflicted significant economic losses on China's poultry industry. Rapid and accurate detection of DTMUV is crucial for effective prevention and control measures. In this study, we developed a novel, rapid visual detection assay that combines reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) with the CRISPR/Cas12a system for on-site detection of DTMUV. Our results demonstrate that this assay can sensitively and specifically detect the specific DNA plasmids containing the DTMUV

Indexed as

Cas12aDuck Tembusu virusRT-LAMP

Identifiers

PMID39682403
PMCPMC11640670

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.