ArticleThe Journal of cell biology1985
Alpha-actinin from sea urchin eggs: biochemical properties, interaction with actin, and distribution in the cell during fertilization and cleavage.
Article in The Journal of cell biology, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 82 citations in OpenAlex.
- Human Plastins are Novel Cytoskeletal pH Sensors with a Reduced F-actin Bundling Capacity at Basic pH.Journal of molecular biology · 2025Article
- Cytokinetic contractile ring structural progression in an early embryo: positioning of scaffolding proteins, recruitment of α-actinin, and effects of myosin II inhibition.Frontiers in cell and developmental biology · 2024Article
- Cellular and molecular aspects of oocyte maturation and fertilization: a perspective from the actin cytoskeleton.Zoological letters · 2020Review
- The F-actin bundler α-actinin Ain1 is tailored for ring assembly and constriction during cytokinesis in fission yeast.Molecular biology of the cell · 2016Article
- Article
- Cell-sized spherical confinement induces the spontaneous formation of contractile actomyosin rings in vitro.Nature cell biology · 2015Article
- An equatorial contractile mechanism drives cell elongation but not cell division.PLoS biology · 2014Article
- α-Actinin and fimbrin cooperate with myosin II to organize actomyosin bundles during contractile-ring assembly.Molecular biology of the cell · 2012Article
- The domain structure of Entamoeba α-actinin2.Cellular & molecular biology letters · 2010Article
- Mechanisms of contractile-ring assembly in fission yeast and beyond.Seminars in cell & developmental biology · 2010Article
- Alpha-actinin is required for tightly regulated remodeling of the actin cortical network during cytokinesis.Developmental cell · 2007Article
- Roles of a fimbrin and an alpha-actinin-like protein in fission yeast cell polarization and cytokinesis.Molecular biology of the cell · 2001Article
- Actin-bundling protein isolated from pollen tubes of lily. Biochemical and immunocytochemical characterizationPlant physiology · 1998Article
- Calcium release at fertilization in starfish eggs is mediated by phospholipase Cgamma.The Journal of cell biology · 1997Article
- Radixin, a barbed end-capping actin-modulating protein, is concentrated at the cleavage furrow during cytokinesis.The Journal of cell biology · 1991Article
- Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene.American journal of human genetics · 1990Article
- Low ionic strength solubility of myosin in sea urchin egg extracts is mediated by a myosin-binding protein.The Journal of cell biology · 1987Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A protein similar to alpha-actinin has been isolated from unfertilized sea urchin eggs. This protein co-precipitated with actin from an egg extract as actin bundles. Its apparent molecular weight was estimated to be approximately 95,000 on an SDS gel: it co-migrated with skeletal-muscle alpha-actinin. This protein also co-eluted with skeletal muscle alpha-actinin from a gel filtration column giving a Stokes radius of 7.7 nm, and its amino acid composition was very similar to that of alpha-actinins. It reacted weakly but significantly with antibodies against chicken skeletal muscle alpha-actinin. We designated this protein as sea urchin egg alpha-actinin. The appearance of sea urchin egg alpha-actinin as revealed by electron microscopy using the low-angle rotary shadowing technique was also similar to that of skeletal muscle alpha-actinin. This protein was able to cross-link actin filaments side by side to form large bundles. The action of sea urchin egg alpha-actinin on the actin filaments was studied by viscometry at a low-shear rate. It gelled the F-actin solution at a molar ratio to actin of more than 1:20, at pH 6-7.5, and at Ca ion concentration less than 1 microM. The effect was abolished by the presence of tropomyosin. Distribution of this protein in the egg during fertilization and cleavage was investigated by means of microinjection of the rhodamine-labeled protein in the living eggs. This protein showed a uniform distribution in the cytoplasm in the unfertilized eggs. Upon fertilization, however, it was concentrated in the cell cortex, including the fertilization cone. At cleavage, it seemed to be concentrated in the cleavage furrow region.
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