Evidence map›Paper›PMID 39680383›Full record

ArticleThe Journal of antimicrobial chemotherapy2025

Genomic analysis of early ST32 Acinetobacter baumannii strains recovered in US military treatment facilities reveals distinct lineages and links to the origins of the Tn6168 ampC transposon.

Liam A Tobin, Eradah Abu Sabah, Francois Lebreton, Garry S A Myers, Patrick T McGann, Mehrad Hamidian

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Article in The Journal of antimicrobial chemotherapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. The (un)successful global spread ofMicrobial genomics · 2026
    Article
  2. Lineage-specific loss of the type VI secretion system inAntimicrobial agents and chemotherapy · 2026
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Liam A TobinAustralian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, NSW 2007, Australia.
Eradah Abu SabahAustralian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, NSW 2007, Australia.
Francois LebretonMultidrug Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Garry S A MyersAustralian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, NSW 2007, Australia.
Patrick T McGannMultidrug Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, MD, USA.ORCID 0000-0003-1548-9438
Mehrad HamidianAustralian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, NSW 2007, Australia.ORCID 0000-0002-3614-7261

Funding

Australian Government Research Training ProgramAustralian Institute for MicrobiologyMinistry of EducationUnited States Defense Health Program
6 · The paper itself

Abstract

objectivesTo study the population structure and genomic characteristics, including antimicrobial resistance genes, plasmid types and surface polysaccharide type, of the globally distributed Acinetobacter baumannii belonging to ST32 (Institut Pasteur scheme).

methodsAntibiotic resistance phenotype for 19 antibiotics was determined using Vitek 2. Whole-genome sequencing was performed using the Illumina MiSeq platform. Genomes were assembled using Newbler. Phylogenetic analysis was done by determining the core-genome alignments using Panaroo v1.3, analysed in IQ-Tree2 v2.2.0.3 to construct Maximum Likelihood trees using the RaxML software. Resistance genes and IS were identified using the Abricate programme, and ISFinder databases.

resultsOne hundred and thirty-three (n = 133) ST32 A. baumannii isolates were analysed in this study. These genomes originated mainly from US military treatment facilities (n = 113), but also included additional publicly available genomes in GenBank (n = 20) recovered from a broad geographic distribution extending to Asia and South America. Phylogenetic analysis of all 133 genomes revealed at least four clades, with over 80 genomes forming a tightly clustered branch, suggesting they are likely to represent outbreak strains. Analysis of the ampC region showed that ST32 strains played a significant role in the formation of the widely distributed ampC transposon, Tn6168, and supplying DNA segments containing an ISAba1-ampC from ST32s via homologous recombination.

conclusionsST32 strains played a significant role in the evolution of antibiotic resistance in several widely distributed sequence types including ST1 (global clone 1) and ST3.

Indexed as

Acinetobacter baumanniiAcinetobacter InfectionsBacterial Proteinsbeta-LactamasesDNA Transposable ElementsGenome, BacterialAnti-Bacterial AgentsDrug Resistance, Multiple, BacterialGenomicsHumansMicrobial Sensitivity TestsPhylogenyPlasmidsUnited StatesWhole Genome SequencingAmpC beta-lactamasesAnti-Bacterial AgentsBacterial Proteinsbeta-LactamasesDNA Transposable Elements

Identifiers

PMID39680383
PMCPMC11879209

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.