Evidence map›Paper›PMID 39680170›Full record

ArticleApplied microbiology and biotechnology2024

Improved recombinant expression of soluble cathepsin B and L in Escherichia coli.

Christina Möller, Niklas Rimkus, Ferdinand F O Skala, Maëlle Merouze, Dominique Böttcher, Mark Dörr, Uwe T Bornscheuer

Abstract read
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In one paragraph

Article in Applied microbiology and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Christina MöllerInstitute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, University of Greifswald, Greifswald, Germany.ORCID http://orcid.org/0000-0002-8286-1589
Niklas RimkusInstitute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, University of Greifswald, Greifswald, Germany.ORCID http://orcid.org/0009-0002-4057-2057
Ferdinand F O SkalaInstitute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, University of Greifswald, Greifswald, Germany.ORCID http://orcid.org/0009-0001-7028-3427
Maëlle MerouzeInstitute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, University of Greifswald, Greifswald, Germany.ORCID http://orcid.org/0009-0000-5840-7004
Dominique BöttcherInstitute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, University of Greifswald, Greifswald, Germany.ORCID http://orcid.org/0000-0001-9981-014X
Mark DörrInstitute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, University of Greifswald, Greifswald, Germany.ORCID http://orcid.org/0000-0003-3270-6895
Uwe T BornscheuerInstitute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, University of Greifswald, Greifswald, Germany. uwe.bornscheuer@uni-greifswald.de.ORCID http://orcid.org/0000-0003-0685-2696

Funding

Deutsche Forschungsgemeinschaft GRK 2719
6 · The paper itself

Abstract

Cysteine cathepsins such as cathepsin B and L play an important role in numerous diseases like acute pancreatitis or SARS-CoV-2 and therefore have high potential for the development of new therapeutics. To be able to screen for potent and selective inhibitors sufficient amounts of protein are required. Here, we present an easy and efficient protocol for the recombinant expression of soluble and active murine cathepsin B and L. For this, we used the strain E. coli SHuffle® T7 Express which is capable of forming disulfide bridges in the cytoplasm. The enzymes were purified by immobilized nickel ion-affinity chromatography. Using different constructs and media, expression levels were significantly improved and expression yields of 80 ± 2 mg L

Indexed as

Cathepsin BCathepsin LEscherichia coliRecombinant ProteinsAnimalsGene ExpressionKineticsMiceMutagenesis, Site-DirectedSolubilityCathepsin BCathepsin LCtsb protein, mouseCtsl protein, mouseRecombinant ProteinsCathepsin BCathepsin LE. coli SHuffle® T7 ExpressRecombinant gene expression

Identifiers

PMID39680170
PMCPMC11649755

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.