Evidence map›Paper›PMID 39677634›Full record

ArticlebioRxiv : the preprint server for biology2024

Distinct differentiation trajectories leave lasting impacts on gene regulation and function of V2a interneurons.

Nicholas H Elder, Alireza Majd, Emily A Bulger, Ryan M Samuel, Lyandysha V Zholudeva, Todd C McDevitt, Faranak Fattahi

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Nicholas H ElderDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
Alireza MajdDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
Emily A BulgerDevelopmental and Stem Cell Biology Graduate Program, University of California, San Francisco, CA 94158, USA.
Ryan M SamuelDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
Lyandysha V ZholudevaGladstone Institute of Cardiovascular Disease, Gladstone Institutes, San Francisco, CA 94158, USA.
Todd C McDevittGladstone Institute of Cardiovascular Disease, Gladstone Institutes, San Francisco, CA 94158, USA.
Faranak FattahiDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.ORCID 0000-0002-9473-8596

Funding

PREDOCTORAL TRAINING IN DEVELOPMENTAL BIOLOGYT32HD007470 · NICHD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Todd Nystul · 1994 to 2026
$7.7M
Modeling Neurodegenerative Disease Manifestations in Human Enteric NeuronsDP2NS116769 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FATTAHI, FARANAK · 2019 to 2019
$2.4M
Human pluripotent stem cells for the study of gastrointestinal dysmotilityR01DK121169 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FATTAHI, FARANAK · 2020 to 2024
$1.8M
Illumina NovaSeq 6000 Sequencing SystemS10OD028511 · OD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CHOW, ERIC D · 2020 to 2020
$583k
NICHD NIH HHS T32 HD007470NIDDK NIH HHS R01 DK121169NIH HHS S10 OD028511NINDS NIH HHS DP2 NS116769
6 · The paper itself

Abstract

During development, early regionalization segregates lineages and directs diverse cell fates. Sometimes, however, distinct progenitors produce analogous cell types. For example, V2a neurons, are excitatory interneurons that emerge from different anteroposterior progenitors. V2a neurons demonstrate remarkable plasticity after spinal cord injury and improve motor function, showing potential for cell therapy. To examine how lineage origins shape their properties, we differentiated V2a neurons from hPSC-derived progenitors with distinct anteroposterior identities. Single-nucleus multiomic analysis revealed lineage-specific transcription factor motifs and numerous differentially expressed genes related to axon growth and calcium handling. Bypassing lineage patterning via transcription factor-induced differentiation yielded neurons distinct from both developmentally relevant populations and human tissue, emphasizing the need to follow developmental steps to generate authentic cell identities. Using

Identifiers

PMID39677634
PMCPMC11642877

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.