Evidence map›Paper›PMID 39675942›Full record

ArticleBiosensors & bioelectronics2025

Sample-to-answer detection of miRNA from whole blood using thermally responsive alkane partitions.

Evan H Benke, David J Boegner, Alejandra M Bogusch, Ian M White

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Evan H BenkeFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
David J BoegnerFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Alejandra M BoguschFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Ian M WhiteFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA. Electronic address: ianwhite@umd.edu.

Funding

A rapid, automated system for bacteria profiling of intra-abdominal infectionsR01AI153564 · NIAID · UNIV OF MARYLAND, COLLEGE PARK · PI DEVOE, DON L, WHITE, IAN M · 2021 to 2025
$2.7M
Enabling exosome biomarker development via digitized single vesicle analysisR01GM130923 · NIGMS · UNIV OF MARYLAND, COLLEGE PARK · PI DEVOE, DON L, WHITE, IAN M · 2019 to 2022
$1.7M
NIAID NIH HHS R01 AI153564NIGMS NIH HHS R01 GM130923
6 · The paper itself

Abstract

Circulating miRNA offers a tremendous opportunity as a biomarker paradigm for many applications in disease diagnostics, including point-of-care diagnostics for global health needs. However, despite the numerous miRNA detection schemes reported, there still does not exist a solution for highly sensitive sample-to-answer detection of miRNA directly from complex samples, such as whole blood. We recently developed thermally responsive alkane partitions (TRAPs), which - when combined with magnetic microbeads - enable the complete assay automation from whole blood. Here we apply TRAPs with ligation-LAMP to automate the detection of miRNA in whole blood samples. MBs and a TRAP enable the automated purification of miRNA from blood, while a novel displacement-ligation method is utilized to trigger the ligation-LAMP reaction, which is streamlined into one step by a second TRAP. Using easily manufacturable TRAP-enabled assay cassettes and a custom low-cost handheld instrument, we report the specific detection of miR-155 at concentrations as low as 15 fM in whole blood with no intermediate steps by the user. This new approach creates the opportunity for point-of-care miRNA-based diagnostics with global health applications.

Indexed as

AlkanesBiosensing TechniquesMicroRNAsEquipment DesignHumansLimit of DetectionMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPoint-of-Care SystemsTemperatureAlkanesMicroRNAsMIRN155 microRNA, humanLigation LAMPmiRNAPoint-of-care diagnostics

Identifiers

PMID39675942
PMCPMC11700762

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.