Polystyrene nanoplastics impact the bioenergetics of developing zebrafish and limit molecular and physiological adaptive responses to acute temperature stress.

Plastic pollution is a growing environmental concern due to its ubiquitous impact on aquatic ecosystems. Nanoplastics can be generated from the breakdown of plastic waste and interact with organisms at the cellular level, potentially disrupting cellular physiology. We investigated the effects of 44 nm polystyrene nanoparticles (44 nm NanoPS) on the development and physiology of zebrafish (Danio rerio) in the presence of sublethal heat stress (32 °C vs control, 28 °C). We hypothesized that the simultaneous exposure to nanoplastics and rising temperatures seriously threaten developing fish. This combination could create a critical imbalance: rising temperatures may lead to heightened energy demands, while nanoplastic exposure reduces energy production, threatening animal survival. As expected, 32 °C increased markers associated with animal metabolism and developmental timing, such as growth, hatching, heart rate, and feeding. Changes in apoptosis dynamics, oxygen consumption rates, and a decrease in mitochondrial content were detected as adaptive processes to temperature. 44 nm NanoPS alone did not alter development but decreased mitochondrial efficiency in ATP production and increased apoptosis in the heart. Surprisingly, exposure to 44 nm NanoPS at 32 °C did not cause major implications to survival, developmental success, or morphology. Still, 44 nm NanoPS mitigated the temperature-driven change in heart rate, increased oxidative stress, and decreased the coupling efficiency of the less abundant and highly active mitochondria under heat stress. We highlight the interplay between temperature and nanoplastics exposure and suggest that the combined impact of nanoplastics and temperature stress results in a scenario where physiological adaptations are strained, potentially leading to compromised development. This research underscores the need for further investigation into the metabolic costs of plastic pollution, particularly in the context of global warming, to better understand its long-term implications for aquatic life.