Evidence map›Paper›PMID 39674089›Full record

ArticleEBioMedicine2025

Assessing the kinetics of oxygen-unloading from red cells using FlowScore, a flow-cytometric proxy of the functional quality of blood.

Julija Rabcuka, Peter A Smethurst, Katharina Dammert, Jarob Saker, Gemma Aran, Geraldine M Walsh, Joanne C G Tan, Margarita Codinach, Ken McTaggart, Denese C Marks and 9 more

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Article in EBioMedicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Julija RabcukaDepartment of Physiology, Anatomy & Genetics, University of Oxford, Oxford, UK.
Peter A SmethurstComponent Development Laboratory, NHS Blood and Transplant, Cambridge, UK.
Katharina DammertSysmex Europe SE, Bornbarch 1, Norderstedt, 22848, Germany.
Jarob SakerSysmex Europe SE, Bornbarch 1, Norderstedt, 22848, Germany.
Gemma AranCell Laboratory, Banc de Sang i Teixits, Barcelona, Spain.
Geraldine M WalshProduct and Process Development, Canadian Blood Services, Vancouver, Canada.
Joanne C G TanResearch and Development, Australian Red Cross Lifeblood, Sydney, Australia; Sydney Medical School, University of Sydney, Camperdown, Australia.
Margarita CodinachCell Laboratory, Banc de Sang i Teixits, Barcelona, Spain; Transfusional Medicine Group, Vall d'Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain.
Ken McTaggartProduct and Process Development, Canadian Blood Services, Ottawa, Canada.
Denese C MarksResearch and Development, Australian Red Cross Lifeblood, Sydney, Australia; Sydney Medical School, University of Sydney, Camperdown, Australia.
Stephan J L BakkerDepartment of Internal Medicine, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
Amy McMahonBritish Heart Foundation Cardiovascular Epidemiology Unit, Department of Public Health & Primary Care, University of Cambridge, UK; Victor Phillip Dahdaleh Heart and Lung Research Institute, University of Cambridge, Cambridge, UK; National Institute for Health and Care Research Blood and Transplant Research Unit in Donor Health and Behaviour, University of Cambridge, Cambridge, UK.
Emanuele Di AngelantonioBritish Heart Foundation Cardiovascular Epidemiology Unit, Department of Public Health & Primary Care, University of Cambridge, UK; Victor Phillip Dahdaleh Heart and Lung Research Institute, University of Cambridge, Cambridge, UK; National Institute for Health and Care Research Blood and Transplant Research Unit in Donor Health and Behaviour, University of Cambridge, Cambridge, UK; British Heart Foundation Centre of Research Excellence, University of Cambridge, Cambridge, UK; Health Data Research UK Cambridge, Wellcome Genome Campus and University of Cambridge, Cambridge, UK; Health Data Science Research Centre, Human Technopole, Milan, Italy.
David J RobertsNHS Blood and Transplant, John Radcliffe Hospital, Oxford, UK; Radcliffe Department of Medicine, University of Oxford, Oxford, UK.
Slawomir BlonskiInstitute of Fundamental Technological Research Polish Academy of Sciences, Warsaw, Poland.
Piotr M KorczykInstitute of Fundamental Technological Research Polish Academy of Sciences, Warsaw, Poland.
Atsushi ShirakamiMedical and Scientific Affairs, Sysmex Corporation, Kobe, Japan.
Rebecca CardiganComponent Development Laboratory, NHS Blood and Transplant, Cambridge, UK; Department of Haematology, University of Cambridge, UK.
Pawel SwietachDepartment of Physiology, Anatomy & Genetics, University of Oxford, Oxford, UK. Electronic address: pawel.swietach@dpag.ox.ac.uk.

Funding

Medical Research Council MC_PC_17230Medical Research Council MR/L003120/1
6 · The paper itself

Abstract

backgroundMetrics evaluating the functional quality of red blood cells (RBCs) must consider their role in oxygen delivery. Whereas oxygen-carrying capacity is routinely reported using haemoglobin assays, the rate of oxygen exchange is not measured, yet also important for tissue oxygenation. Since oxygen-unloading depends on the diffusion pathlength inside RBCs, cell geometry offers a plausible surrogate.

methodsWe related the time-constant of oxygen-unloading (τ), measured using single-cell oxygen saturation imaging, with flow-cytometric variables recorded on a haematology analyser. Experiments compared freshly-drawn RBCs with stored RBCs, wherein metabolic run-down and spherical remodelling hinder oxygen unloading.

findingsMultivariable regression related τ to a ratio of side- and forward-scatter, referred to herein as FlowScore. FlowScore was able to distinguish, with sensitivity and specificity >80%, freshly drawn blood from blood that underwent storage-related kinetic attrition in O

interpretationWe establish FlowScore as a widely-accessible and cost-effective surrogate of RBC oxygen-unloading kinetics. As a metric of a cellular process that is sensitive to storage and disease, we propose FlowScore as an RBC quality marker for blood-banking and haematology.

fundingSee Acknowledgements.

Indexed as

ErythrocytesFlow CytometryOxygen2,3-DiphosphoglycerateAdultBlood PreservationFemaleHumansKineticsMaleMiddle Aged2,3-DiphosphoglycerateOxygenAssayErythrocytesHaematologyOxygen transportStorage lesionTransfusion

Identifiers

PMID39674089
PMCPMC11730303

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.