Evidence map›Paper›PMID 39673958›Full record

ArticleBiosensors & bioelectronics2025

Quencher-free CRISPR-based molecular detection using an amphiphilic DNA fluorescence probe.

Rui Yang, Xin Guan, Jiongyu Zhang, Jeong Moon, Chong Guo, Zhengyang Jia, Chengyu Hou, Uyanga Ganbaatar, Steven Tricarico, Anson W K Ma and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. CRISPR-Programmable Nanoparticle Transport in Salt-Gated Porous Media for Visual DNA Detection.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2026
    Article
  2. Review
  3. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Rui YangDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, United States; Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, United States.
Xin GuanDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, United States; Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, United States.
Jiongyu ZhangDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, United States.
Jeong MoonDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, United States.
Chong GuoDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, United States; Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, United States.
Zhengyang JiaDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, United States.
Chengyu HouDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, United States; Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, United States.
Uyanga GanbaatarDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, United States; Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, United States.
Steven TricaricoDepartment of Chemical Engineering, College of Engineering, University of Connecticut, Storrs, CT, 06269, United States.
Anson W K MaDepartment of Chemical Engineering, College of Engineering, University of Connecticut, Storrs, CT, 06269, United States; Polymer Program, Institute of Materials Science, University of Connecticut, Storrs, CT, 06269, United States.
Changchun LiuDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, United States. Electronic address: chaliu@uchc.edu.

Funding

Low-Cost CRISPR-on-Paper for Cervical Cancer Screening at the Point of CareU01CA269147 · NCI · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI Changchun Liu · 2022 to 2026
$2.6M
NCI NIH HHS U01 CA269147
6 · The paper itself

Abstract

Rapid, sensitive, and specific nucleic acid detection methods play crucial roles in clinical diagnostics and healthcare. Here, we report a novel amphiphilic DNA fluorescence probe for CRISPR-based nucleic acid detection. Unlike conventional fluorophore-quencher probe detection system, our amphiphilic DNA fluorescence probe features a hydrophobic Cy5 fluorophore head and a hydrophilic single-stranded DNA (ssDNA) tail. By combining the amphiphilic DNA fluorescence probe with a paper-based microfluidic device, we developed a quencher-free, CRISPR-based detection system for target nucleic acid quantification. In the presence of the target nucleic acid, the activated CRISPR-Cas12a enzyme cleaves the hydrophilic ssDNA tail of the amphiphilic DNA fluorescence probe, releasing the hydrophobic Cy5 head and altering the wettability of the CRISPR reaction solution. When the CRISPR reaction product is applied to the paper-based microfluidic device, the migration of the cleaved Cy5 head along the hydrophilic microfluidic channel is limited. The higher the target nucleic acid concentration, the shorter the fluorescence diffusion distance, enabling visual quantification of the nucleic acid target. We used human papillomavirus-16 (HPV-16) DNA as a model to evaluate the analytical performance of the system. Furthermore, we validated its clinical feasibility by testing clinical swab samples, achieving results comparable to the traditional PCR method. Our quencher-free CRISPR-based detection system shows potential for simple, affordable, and sensitive clinical diagnostics of HPV-associated cancer and other infectious diseases.

Indexed as

Biosensing TechniquesCRISPR-Cas SystemsDNA, ViralFluorescent DyesHuman papillomavirus 16Bacterial ProteinsCarbocyaninesClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Associated ProteinsDNA ProbesDNA, Single-StrandedEndodeoxyribonucleasesHumansLab-On-A-Chip DevicesPapillomavirus InfectionsBacterial ProteinsCarbocyaninesCas12a proteinCRISPR-Associated Proteinscyanine dye 5DNA ProbesDNA, Single-StrandedDNA, ViralEndodeoxyribonucleasesFluorescent DyesAmphiphilic DNA fluorescence probeCRISPR-Cas12a detectionHuman papillomavirus (HPV)-associated cancer screeningNucleic acid quantificationRecombinase polymerase amplification

Identifiers

PMID39673958
PMCPMC11700774

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.