Evidence map›Paper›PMID 39671565›Full record

ArticleG3 (Bethesda, Md.)2025

High quality genome assembly and annotation (v1) of the eukaryotic freshwater microalga Coccomyxa elongata SAG 216-3b.

Anton Kraege, Edgar Chavarro-Carrero, Eva Schnell, Stefanie Heilmann-Heimbach, Kerstin Becker, Karl Köhrer, Bruno Huettel, Nafiseh Sargheini, Philipp Schiffer, Ann-Marie Waldvogel and 2 more

Abstract read
In one paragraph

Article in G3 (Bethesda, Md.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Anton KraegeInstitute of Plant Sciences, Department of Biology, University of Cologne, Zülpicher Straße 47b, Cologne 50674, Germany.ORCID 0000-0002-4741-5671
Edgar Chavarro-CarreroInstitute of Plant Sciences, Department of Biology, University of Cologne, Zülpicher Straße 47b, Cologne 50674, Germany.
Eva SchnellInstitute of Plant Sciences, Department of Biology, University of Cologne, Zülpicher Straße 47b, Cologne 50674, Germany.
Stefanie Heilmann-HeimbachInstitute of Human Genetics, University Hospital of Bonn, University of Bonn, Venusberg, Sigmund-Freund, Straße 25, Bonn 53127, Germany.
Kerstin BeckerCologne Center for Genomics (CCG), Medical Faculty, University of Cologne, Weyertal 115b, Cologne 50931, Germany.
Karl KöhrerBiological and Medical Research Centre (BMFZ), Genomics and Transcriptomics Laboratory, Heinrich-Heine-University Düsseldorf, Universitätsstraße 1, Düsseldorf 40225, Germany.
Bruno HuettelMax Planck Genome Centre, Max Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, Cologne 50829, Germany.
Nafiseh SargheiniMax Planck Genome Centre, Max Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, Cologne 50829, Germany.
Philipp SchifferInstitute of Zoology, Department of Biology, University of Cologne, Zülpicher Straße 47b, Cologne 50674, Germany.ORCID 0000-0001-6776-0934
Ann-Marie WaldvogelInstitute of Zoology, Department of Biology, University of Cologne, Zülpicher Straße 47b, Cologne 50674, Germany.
Bart P H J ThommaInstitute of Plant Sciences, Department of Biology, University of Cologne, Zülpicher Straße 47b, Cologne 50674, Germany.
Hanna RovenichInstitute of Plant Sciences, Department of Biology, University of Cologne, Zülpicher Straße 47b, Cologne 50674, Germany.ORCID 0000-0001-6138-4480

Funding

Alexander von Humboldt FoundationDeutsche Forschungsgemeinschaft 458090666.DFG ENP 434028868German Federal Ministry of Education and Research and is furthermore supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) 390686111
6 · The paper itself

Abstract

Unicellular green algae of the genus Coccomyxa are recognized for their worldwide distribution and ecological versatility. Coccomyxa elongata is a freshwater species of the Coccomyxa simplex clade, which also includes lichen symbionts. To facilitate future molecular and phylogenomic studies of this versatile clade of algae, we generated a high-quality genome assembly for C. elongata Chodat & Jaag SAG 216-3b within the framework of the Biodiversity Genomics Center Cologne (BioC2) initiative. A combination of long-read PacBio HiFi and Oxford Nanopore Technologies with chromatin conformation capture (Hi-C) sequencing led to the assembly of the genome into 21 scaffolds with a total length of 51.4 Mb and an N50 of 2.8 Mb. Nineteen of the scaffolds represent highly complete nuclear chromosomes delimited by telomeric repeats, while the two additional scaffolds represent the mitochondrial and plastid genomes. Transcriptome-guided gene annotation resulted in the identification of 14,811 protein-coding genes, of which 61% have annotated protein family domains and 841 are predicted to be secreted. Benchmarking universal single-copy orthologs analysis against the Chlorophyta database identified a total of 1,494 (98.4%) complete gene models, suggesting a highly complete genome annotation.

Indexed as

ChlorophyceaeChlorophytaGenome, PlantGenomicsMicroalgaeMolecular Sequence AnnotationFresh WaterPhylogenyCoccomyxagenome annotationgenome assemblylong-read sequencingTrebouxiophyceaeunicellular algae

Identifiers

PMID39671565
PMCPMC11797067

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.