Evidence map›Paper›PMID 39671285›Full record

ArticleSTAR protocols2024

Protocol for murine multi-tissue deep immunophenotyping using a 40-color full-spectrum flow cytometry panel.

Pierre Lemaitre, Chantal Mathieu, Conny Gysemans

Abstract read
In one paragraph

Article in STAR protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Pierre LemaitreClinical and Experimental Endocrinology (CEE), CHROMETA, KU Leuven, Campus Gasthuisberg O&N1bis, Leuven, Belgium. Electronic address: pierre.lemaitre@kuleuven.be.
Chantal MathieuClinical and Experimental Endocrinology (CEE), CHROMETA, KU Leuven, Campus Gasthuisberg O&N1bis, Leuven, Belgium.
Conny GysemansClinical and Experimental Endocrinology (CEE), CHROMETA, KU Leuven, Campus Gasthuisberg O&N1bis, Leuven, Belgium. Electronic address: conny.gysemans@kuleuven.be.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The innate and adaptive immune systems, though often studied separately, interact deeply and respond to stimuli simultaneously, with leukocytes displaying a range of pro- to anti-inflammatory phenotypes. This protocol details a procedure for characterizing murine innate and adaptive immune phenotypes using a 40-color full-spectral flow cytometry panel. We describe steps for organ collection, sample preparation, immunofluorescent staining, and acquisition to reproducibly and cost-effectively study tissue-resident leukocytes, their subpopulations, and inflammatory status in various organs.

Indexed as

Flow CytometryImmunophenotypingAdaptive ImmunityAnimalsImmunity, InnateLeukocytesMiceantibodycell isolationflow cytometryimmunology

Identifiers

PMID39671285
PMCPMC11697560

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.