ArticleEcology and evolution2024
Quantifying the Detection Sensitivity and Precision of qPCR and ddPCR Mechanisms for eDNA Samples.
Article in Ecology and evolution, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Passive Water Filtration Enhances the Detection of Endangered Fish Species in Rivers:Ecology and evolution · 2026Article
- FishChip: Development of a High-Throughput qPCR Assay for Freshwater Fish eDNA Quantification and Its Comparison With Droplet Digital PCR.Molecular ecology resources · 2026Article
- Review
- From Gene Copies to Cell Numbers: Advancing Quantitative Approaches in Protistan Ecology Using Digital PCR.Molecular ecology resources · 2026Article
- Operationalising Environmental DNA (eDNA) Detection of Major Malaria Vector Species in Ghana.Molecular ecology resources · 2026Article
- The potential of methylation signal detection in eDNA toward functional ecological monitoring.Communications biology · 2026Review
- One Assay to Rule Them All: Development of a Global Environmental DNA Tool to Support Range-Wide Surveys of an Imperiled Sawfish and Its Application in the Eastern Tropical Pacific.Ecology and evolution · 2026Article
- Progress of Rapid Detection Technology for Aquatic Microorganisms: A Comprehensive Review.Microorganisms · 2026Review
- From rivers to the sea: a single gene assay for New Zealand freshwater eel eDNA monitoring.PeerJ · 2026Article
- Environmental DNA (eDNA) Technology in Biodiversity and Ecosystem Health Research: Advances and Prospects.Ecology and evolution · 2026Review
- Pattern of Detections Across Multiple Environmental Messenger RNAs (e-mRNAs) in Stressor-Exposed Zebrafish (Ecology and evolution · 2026Article
- Advances in rapid detection technologies for zoonotic diseases: a one health-oriented review.Frontiers in cellular and infection microbiology · 2026Review
- Analyzing qPCR data: Better practices to facilitate rigor and reproducibility.Biochemistry and biophysics reports · 2025Article
- Passive air sampling detects environmental DNA transfer from water into air.Scientific reports · 2025Article
- Rapid Detection of Animal-Derived Components in Plant-Based Meat Alternatives Using Recombinase Polymerase Amplification.Foods (Basel, Switzerland) · 2025Article
- Rapid DNA/eDNA-Based ID Tools for Improved Chondrichthyan Monitoring and Management.Molecular ecology resources · 2025Review
- Extirpation of Lake Sturgeon in an Ontario Lake Following Dam Construction and Watershed Diversion Confirmed by Indigenous Traditional Knowledge and Sedimentary eDNA.Ecology and evolution · 2025Article
- Comparing the precision of two digital PCR applications for copy number comparisons in protists.Scientific reports · 2025Article
- Differential decay of multiple environmental nucleic acid components.Scientific reports · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental DNA (eDNA) detection employing quantitative PCR (qPCR) and droplet digital PCR (ddPCR) offers a non-invasive and efficient approach for monitoring aquatic organisms. Accurate and sensitive quantification of eDNA is crucial for tracking rare and invasive species and understanding the biodiversity abundance and distribution of aquatic organisms. This study compares the sensitivity and quantification precision of qPCR and ddPCR for eDNA surveys through Bayesian inference using latent parameters from both known concentration (standards) and environmental samples across three teleost fish species assays. The results show that ddPCR offers higher sensitivity and quantification precision, particularly at low DNA concentrations (< 1 copy/μL reaction), than qPCR. These findings highlight the superior performance of ddPCR for eDNA detection at low concentrations, guiding researchers towards more reliable methods for effective species monitoring. Additionally, this study indicates that a two-step (detection and concentration) model increased the precision of qPCR results, useful for enhancing the robustness of eDNA quantification. Furthermore, we investigated the lower limit of quantification for ddPCR, providing insights on how such limit can be extended, which could also be applied to qPCR.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.