Evidence map›Paper›PMID 39668303›Full record

ArticleClinical rheumatology2025

Downregulated YTHDF2 expression in systemic lupus erythematosus is associated with inflammatory and neutrophil cytokine production.

Hongshuai Zhao, Mengfan Lan, Zikun Huang, Peng Fu, Biqi Fu, Yang Guo, Junming Li, Qing Luo

Abstract read
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In one paragraph

Article in Clinical rheumatology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
  3. mFrontiers in immunology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hongshuai Zhao *Department of Clinical Laboratory, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, No.17, YongwaiZhengjie, Donghu District, Nanchang, 330006, Jiangxi, China.ORCID http://orcid.org/0009-0001-2023-124X
Mengfan Lan *Department of Clinical Laboratory, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, No.17, YongwaiZhengjie, Donghu District, Nanchang, 330006, Jiangxi, China.
Zikun Huang *Department of Clinical Laboratory, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, No.17, YongwaiZhengjie, Donghu District, Nanchang, 330006, Jiangxi, China.
Peng FuDepartment of Clinical Laboratory, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, No.17, YongwaiZhengjie, Donghu District, Nanchang, 330006, Jiangxi, China.
Biqi FuDepartment of Rheumatology, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330006, Jiangxi, China.
Yang GuoDepartment of Clinical Laboratory, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, No.17, YongwaiZhengjie, Donghu District, Nanchang, 330006, Jiangxi, China.
Junming LiDepartment of Clinical Laboratory, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, No.17, YongwaiZhengjie, Donghu District, Nanchang, 330006, Jiangxi, China. ndyfy2140@ncu.edu.cn.
Qing LuoDepartment of Clinical Laboratory, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, No.17, YongwaiZhengjie, Donghu District, Nanchang, 330006, Jiangxi, China. lxc042@163.com.

Funding

Jiangxi Provincial Natural Science Foundation of China 20212ACB216006Jiangxi Provincial Natural Science Foundation of China 20224BAB216038Jiangxi Provincial Natural Science Foundation of China 20242BAB23077National Natural Science Foundation of China 82160307National Natural Science Foundation of China 82160308
6 · The paper itself

Abstract

objectivesAn increasing body of evidence suggests that N6-methyladenosine (m6A) plays a crucial role in the etiology of SLE. We focused on YTHDF2 expression in neutrophils and the relationship between YTHDF2 and the pathogenesis of SLE.

methodsSixty-one patients with SLE and 48 healthy controls (HC) were recruited, and their clinical characteristics were recorded. The mRNA levels of m6A "writers" (METTL3, METTL14, WTAP), "erasers" (FTO and ALKBH5), "readers" (YTHDF2), and inflammatory factors (interleukin-1β, interleukin-6, interleukin-8, and TNF-α) in neutrophils were determined by reverse transcription-quantitative PCR. The protein of YTHDF2 was determined by Western blotting. The correlations between the YTHDF2 in neutrophils of SLE patients and clinical features were examined by Spearman's method. YTHDF2 and TNF-α expression in neutrophils were examined after stimulation by SLE serums.

resultsThe mRNA expression of YTHDF2 in neutrophils was significantly decreased, and the protein level of YTHDF2 in neutrophils was decreased. The mRNA expression of YTHDF2 in neutrophils correlated with L% (r

conclusionThis study indicates that the levels of YTHDF2 in peripheral blood neutrophils may be involved in the pathogenesis of SLE and could be a novel target for diagnosis and therapy. Key points • The mRNA expression of YTHDF2 in neutrophils of SLE and described that decreased mRNA of YTHDF2 in neutrophils correlated with L%, LMR, WBC, N, N%, NLR, dNLR, and SII. • The mRNA expression of TNF-α in neutrophils was significantly increased and correlated with YTHDF2 in SLE patients. • Neutrophils from health control were significantly downregulated in their YTHDF2 expression and upregulated in their TNF-α expression following stimulation by serum from SLE patients.

Indexed as

CytokinesLupus Erythematosus, SystemicNerve Tissue ProteinsNeutrophilsRNA-Binding ProteinsAdultCase-Control StudiesDown-RegulationFemaleHumansInflammationMaleMiddle AgedRNA, MessengerTumor Necrosis Factor-alphaYoung AdultCytokinesNerve Tissue ProteinsRNA-Binding ProteinsRNA, MessengerTumor Necrosis Factor-alphaYTHDF2 protein, humanN6-MethyladenosineNeutrophilSystemic lupus erythematosusTumor necrosis factor-αYT521-Bhomology domains 2

Identifiers

PMID39668303

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.