Evidence map›Paper›PMID 39666461›Full record

ArticleSTAR protocols2024

Protocol for establishing CRISPR-Cas12a for efficient genome editing of Pseudomonas aeruginosa phages.

Bingjie Yan, Yujia Liu, Yumei Cai, Yuqing Liu, Yibao Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Bingjie YanShandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China; College of Veterinary Medicine, Shandong Agricultural University, Taian, China.
Yujia LiuShandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China; College of Veterinary Medicine, Shandong Agricultural University, Taian, China.
Yumei CaiCollege of Veterinary Medicine, Shandong Agricultural University, Taian, China.
Yuqing LiuShandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China; China-UK Joint Laboratory of Bacteriophage Engineering, Jinan, China; Shandong Vamph Animal Health Products Co., LTD, Jinan, China.
Yibao ChenShandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China; China-UK Joint Laboratory of Bacteriophage Engineering, Jinan, China; Shandong Vamph Animal Health Products Co., LTD, Jinan, China. Electronic address: yibaochen2012@126.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We developed an efficient type V CRISPR-Cas12a system tailored specifically for Pseudomonas aeruginosa phages, showcasing its remarkable cleavage activity and the ability to precisely introduce genetic modifications, including point mutations, deletions, and insertions, into phage genomes. Here, we present a protocol for establishing CRISPR-Cas12a for genome editing of Pseudomonas aeruginosa phages. We describe steps for the construction of pCRISPR-12a plasmid and guide RNA and the utilization of the type V CRISPR-Cas12a system for precise genetic editing of phages. For complete details on the use and execution of this protocol, please refer to Chen et al.

Indexed as

CRISPR-Cas SystemsGene EditingPseudomonas aeruginosaGenome, ViralPlasmidsPseudomonas PhagesRNA, Guide, CRISPR-Cas SystemsRNA, Guide, CRISPR-Cas SystemsCRISPRgenomicsmolecular biology

Identifiers

PMID39666461
PMCPMC11697554

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.