Evidence map›Paper›PMID 39666285›Full record

ArticleJournal of food and drug analysis2023

SSR-based molecular diagnosis for Taiwan tea cultivars and its application in identifying cultivar composition of the processed tea.

Chih-Yi Hu, Hsien-Tsung Tsai, Chui-Feng Chiu, Tsung-Chen Su, Nguyen Hoang Khoi Le, Shu-Dan Yeh

Erratum issuedAbstract readEvaluation Study
In one paragraph

Article in Journal of food and drug analysis, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Chih-Yi HuTea Research and Extension Station, Yangmei, Taoyuan City, 326011, Taiwan.
Hsien-Tsung TsaiTea Research and Extension Station, Yangmei, Taoyuan City, 326011, Taiwan.
Chui-Feng ChiuTea Research and Extension Station, Yangmei, Taoyuan City, 326011, Taiwan.
Tsung-Chen SuTea Research and Extension Station, Yangmei, Taoyuan City, 326011, Taiwan.
Nguyen Hoang Khoi LeDepartment of Life Sciences, National Central University, Taoyuan City, 320317, Taiwan.
Shu-Dan YehDepartment of Life Sciences, National Central University, Taoyuan City, 320317, Taiwan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Taiwan specialty teas are produced with distinct manufacturing processes from specific cultivars of tea plants in Camellia. Due to the widespread transplantation of Taiwan tea cultivars and active international trading of tea materials, an accurate and reliable method to identify tea cultivars at the border is vital to protect the image of premium Taiwan specialty teas. In this study, we introduced the Taiwan Tea Variety Identification (TTVID) kit, a capillary electrophoresis-based multiplex PCR assay consisting of 12 simple sequence repeat (SSR) markers. A database composing these 12 SSR loci genotypes in 144 cultivars was established for marker assessment and molecular diagnosis. The power of discrimination on a locus ranged from 0.7894 to 0.966 and the combined match probability of 12 SSR loci was 5.34e-14. Cultivar pairwise comparison among 144 accessions showed that over 90.6% of the pairs had differential genotypes on at least 10 of 12 SSR loci. Further assessment showed that the TTVID kit could unambiguously recognize the cultivars mixed in the loose-leaf teas processed with various degrees of fermentation and roasting. Our results suggested that this TTVID kit effectively identified cultivar composition in loose-leaf tea and is helpful for border control in preventing adulteration and fraud in the Taiwan tea market.

Indexed as

Camellia sinensisMicrosatellite RepeatsDNA, PlantElectrophoresis, CapillaryGenotypeTaiwanTeaDNA, PlantTea

Identifiers

PMID39666285
PMCPMC10629914

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.