Evidence map›Paper›PMID 39665314›Full record

ArticleJournal of extracellular vesicles2024

Practical advice for extracellular vesicle isolation in plant-microbe interactions: Concerns, considerations, and conclusions.

Hannah Thieron, Laura Krassini, Seomun Kwon, Sebastian Fricke, Sabrine Nasfi, Lorenz Oberkofler, Alessa Ruf, Julia Kehr, Karl-Heinz Kogel, Arne Weiberg and 3 more

Abstract read
In one paragraph

Article in Journal of extracellular vesicles, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Targeting Gli1Stem cell reviews and reports · 2026
    Review
  2. Review
  3. Advances in Research onInternational journal of molecular sciences · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Hannah ThieronUnit for Plant Molecular Cell Biology, Institute for Biology I, RWTH Aachen University, Aachen, Germany.ORCID 0000-0002-0670-7328
Laura KrassiniLMU Munich Biocenter, Ludwig-Maximilian-University of Munich, Munich, Germany.ORCID 0009-0005-7613-910X
Seomun KwonInstitute for Microbiology, Cluster of Excellence on Plant Sciences, Heinrich-Heine University Düsseldorf, Düsseldorf, Germany.ORCID 0000-0002-0514-1465
Sebastian FrickeInstitute of Plant Science and Microbiology, Department of Biology, Universität Hamburg, Hamburg, Germany.ORCID 0009-0004-0859-4437
Sabrine NasfiInstitute of Phytopathology, Research Centre for BioSystems, Land Use and Nutrition, Justus-Liebig-University Giessen, Giessen, Germany.ORCID 0000-0001-5916-0824
Lorenz OberkoflerLMU Munich Biocenter, Ludwig-Maximilian-University of Munich, Munich, Germany.ORCID 0000-0002-9506-2751
Alessa RufLMU Munich Biocenter, Ludwig-Maximilian-University of Munich, Munich, Germany.ORCID 0000-0003-0919-9717
Julia KehrInstitute of Plant Science and Microbiology, Department of Biology, Universität Hamburg, Hamburg, Germany.ORCID 0000-0003-3617-9981
Karl-Heinz KogelInstitute of Phytopathology, Research Centre for BioSystems, Land Use and Nutrition, Justus-Liebig-University Giessen, Giessen, Germany.ORCID 0000-0003-1226-003X
Arne WeibergLMU Munich Biocenter, Ludwig-Maximilian-University of Munich, Munich, Germany.ORCID 0000-0003-4300-4864
Michael FeldbrüggeInstitute for Microbiology, Cluster of Excellence on Plant Sciences, Heinrich-Heine University Düsseldorf, Düsseldorf, Germany.ORCID 0000-0003-0046-983X
Silke RobatzekLMU Munich Biocenter, Ludwig-Maximilian-University of Munich, Munich, Germany.ORCID 0000-0002-9788-322X
Ralph PanstrugaUnit for Plant Molecular Cell Biology, Institute for Biology I, RWTH Aachen University, Aachen, Germany.ORCID 0000-0002-3756-8957

Funding

Deutsche Forschungsgemeinschaft 433194101Deutsche Forschungsgemeinschaft SFB924/3TP15European Research Council 884235
6 · The paper itself

Abstract

In recent years, extracellular vesicles (EVs) have emerged as novel key players in plant-microbe interactions. While it is immensely useful to draw on the established "minimal information for studies of extracellular vesicles" (MISEV) guidelines and precedents in mammalian systems, working with plants and their associated microbes poses specific challenges. To navigate researchers through these obstacles, we offer detailed step-by-step suggestions for those embarking on EV research in the context of plant-microbe interactions. The advice is based on recent publications and our collective experience from the diverse plant and microbe systems studied in a dedicated research consortium. We provide considerations for experimental design, optimization, quality control, and recommendations on how to increase yield, purity, and reproducibility of EV isolation. With this perspective article, we aim not only to assist researchers in our field but also to promote discussions on plant and microbe EVs in the broader EV community.

Indexed as

Extracellular VesiclesHost Microbial InteractionsPlantsapoplastic wash fluidaxenic culturebiological fluidEV isolationEV markerEV quality controlEV size profileplant‐microbe interactions

Identifiers

PMID39665314
PMCPMC11635479

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.