Evidence map›Paper›PMID 39664375›Full record

ArticleFrontiers in immunology2024

T-cell receptor and B-cell receptor repertoires profiling in pleural tuberculosis.

Fengjiao Du, Yunyun Deng, Ling Deng, Boping Du, Aiying Xing, Hong Tao, Hua Li, Li Xie, Xinyong Zhang, Tao Sun and 1 more

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Article in Frontiers in immunology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

11 authors.

Fengjiao Du *National Clinical Laboratory on Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Yunyun Deng *Hangzhou ImmuQuad Biotechnologies, Hangzhou, China.
Ling Deng *Hangzhou ImmuQuad Biotechnologies, Hangzhou, China.
Boping DuNational Clinical Laboratory on Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Aiying XingNational Clinical Laboratory on Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Hong TaoNational Clinical Laboratory on Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Hua LiNational Clinical Laboratory on Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Li XieNational Clinical Laboratory on Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Xinyong ZhangNational Clinical Laboratory on Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Tao SunHangzhou ImmuQuad Biotechnologies, Hangzhou, China.
Hao LiCenter for Infectious Disease Research, School of Medicine, Tsinghua University, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Tuberculosis (TB) is a leading cause of death worldwide from a single infectious agent. In China the most common extra-pulmonary TB (EPTB) is pleural tuberculosis (PLTB). An important clinical feature of PLTB is that the lymphocytes associated with TB will accumulate in the pleural fluid. The adaptive immune repertoires play important roles in Methods: In this study, 10 PLTB patients were enrolled, and their Peripheral Blood Mononuclear Cells(PBMCs) and Pleural Effusion Mononuclear Cells(PEMCs) were collected. After T cells were purified from PBMCs and PEMCs, high-throughput immunosequencing of the TCRβ chain (TRB), TCRγ chain(TRG), and B cell receptor(BCR) immunoglobulin heavy chain (IGH) were conducted on these samples. Results: The TRB, TRG, and BCR IGH repertoires were characterized between the pleural effusion and blood in PLTB patients, and the shared clones were analyzed and collected. The binding activity of antibodies in plasma and pleural effusion to Mtb antigens was tested which indicates that different antibodies responses to Mtb antigens in plasma and pleural effusion in PLTB patients. Moreover, GLIPH2 was used to identify the specificity groups of TRB clusters and Mtb-specific TRB sequences were analyzed and collected by VJ mapping. Conclusion: We characterize the adaptive immune repertoires and identify the shared clones and Mtb-specific clones in pleural effusion and blood in PLTB patients which can give important clues for TB diagnosis, treatment, and vaccine development.

Indexed as

Mycobacterium tuberculosisReceptors, Antigen, B-CellTuberculosis, PleuralAdultAgedB-LymphocytesFemaleHumansMaleMiddle AgedReceptors, Antigen, T-CellReceptors, Antigen, B-CellReceptors, Antigen, T-CellantibodyB cell receptordeep sequencingpleural tuberculosisT cell receptor

Identifiers

PMID39664375
PMCPMC11632106

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