ArticleThe Journal of dermatology2025
The evaluation of Congo red staining combined with fluorescence microscopy in the diagnosis of primary cutaneous amyloidosis.
Article in The Journal of dermatology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Intracellular amyloidosis in peripheral nerve and skeletal muscle.Journal of neuropathology and experimental neurology · 2025Article
- The evaluation of Congo red staining combined with fluorescence microscopy in the diagnosis of primary cutaneous amyloidosis.The Journal of dermatology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Primary cutaneous amyloidosis (PCA) is a chronic pruritic skin disease. The apple-green birefringence of Congo red-stained amyloid under a polarized light microscope (CR-PLM) remains the gold standard in the diagnosis of PCA. However, there are some limitations to this approach. In this study, eighty-two paraffin-embedded biopsy skin samples were collected from patients with a clinical diagnosis of PCA. The sections were respectively stained with hematoxylin-eosin (HE), crystal violet (CV), and Congo red (CR) and observed under a light microscope. CR-stained sections were also observed under a polarized light microscope (CR-PLM) or an ultraviolet (UV)-emitted fluorescence microscope (CR-UFM). Further, 35 cases clinically diagnosed with psoriasis, lichen planus, and prurigo nodularis were selected as the negative control group. The positive rate of amyloid protein detected by CR-UFM (81.71%) was significantly higher than that detected by CR-PLM (70.73%, p = 0.004), CR staining (56.10%, p < 0.001), CV staining (30.49%, p < 0.001), or HE staining (28.05%, p < 0.001). In the control group, 34 (97.14%) cases were negative for amyloid deposits in CR staining, CR-PLM, and CR-UFM sections. The relative number of positive dermal papillae observed by CR-UFM (0.35 ± 0.27) was much more than that observed by CR-PLM (0.15 ± 0.17, p<0.001), CR staining (0.12 ± 0.16, p < 0.001), CV staining (0.07 ± 0.12, p < 0.001), or HE staining (0.05 ± 0.12, p < 0.001). The intensity of fluorescence by CR-UFM was significantly greater than that of the appl-green birefringence by CR-PLM (p < 0.001). Moreover, the amyloid was easily distinguished from the surrounding tissues using the CR-UFM method. In conclusion, the CR-UFM method was superior to CR-PLM, CR staining, CV staining, and HE staining in diagnosing PCA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.