ArticleThe Plant journal : for cell and molecular biology2025
Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery.
Article in The Plant journal : for cell and molecular biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Efficient CRISPR-Cas9 delivery and transgene-free multiplex genome editing in plants using cymbidium mosaic virus-derived vectors.The Plant journal : for cell and molecular biology · 2026Article
- BSMV-mediated genome editing exhibits host-specific heritability: germline transmission in barley and somatic edits in Nicotiana benthamiana.BMC plant biology · 2026Article
- Developmental regulators enable rapid and efficient soybean transformation and CRISPR-mediated genome editing.Plant physiology · 2026Article
- Virus-induced gene editing of stomatal regulators in Nicotiana benthamiana enables rapid functional genomics.The Plant journal : for cell and molecular biology · 2026Article
- Engineering hypercompact IscB nucleases for efficient and versatile genome editing in rice.Genome biology · 2026Article
- Virus-mediated, heritable gene editing in groundcherry (Frontiers in plant science · 2026Article
- Biotechnological and genetic innovations to enhance sorghum adaptation under climate change.Frontiers in plant science · 2026Review
- Viral-mediated delivery of morphogenic regulators enables leaf transformation in Sorghum bicolor (L.).Plant biotechnology journal · 2025Article
- Genome Editing by Grafting.International journal of molecular sciences · 2025Review
- Review
- Virus-Induced Genome Editing (VIGE): One Step Away from an Agricultural Revolution.International journal of molecular sciences · 2025Review
- Review
- RNAi and genome editing of sugarcane: Progress and prospects.The Plant journal : for cell and molecular biology · 2025Review
- Gene editing to enhance biotic stress tolerance in sugarcane.Frontiers in plant science · 2025Article
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Authors and funding
5 authors.
Funding
Abstract
The requirement of in vitro tissue culture for the delivery of gene editing reagents limits the application of gene editing to commercially relevant varieties of many crop species. To overcome this bottleneck, plant RNA viruses have been deployed as versatile tools for in planta delivery of recombinant RNA. Viral delivery of single-guide RNAs (sgRNAs) to transgenic plants that stably express CRISPR-associated (Cas) endonuclease has been successfully used for targeted mutagenesis in several dicotyledonous and few monocotyledonous plants. Progress with this approach in monocotyledonous plants is limited so far by the availability of effective viral vectors. We engineered a set of foxtail mosaic virus (FoMV) and barley stripe mosaic virus (BSMV) vectors to deliver the fluorescent protein AmCyan to track viral infection and movement in Sorghum bicolor. We further used these viruses to deliver and express sgRNAs to Cas9 and Green Fluorescent Protein (GFP) expressing transgenic sorghum lines, targeting Phytoene desaturase (PDS), Magnesium-chelatase subunit I (MgCh), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, orthologs of maize Lemon white1 (Lw1) or GFP. The recombinant BSMV did neither infect sorghum nor deliver or express AmCyan and sgRNAs. In contrast, the recombinant FoMV systemically spread throughout sorghum plants and induced somatic mutations with frequencies reaching up to 60%. This mutagenesis led to visible phenotypic changes, demonstrating the potential of FoMV for in planta gene editing and functional genomics studies in sorghum.
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