ArticleScience advances2024
Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging.
Article in Science advances, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- From spectroscopic analysis to clinical imaging: emerging applications and persistent challenges of raman spectroscopy in CTCs-based liquid biopsies.Annals of medicine · 2026Review
- Ionic Liquid Electrolytes for Extreme Temperature Conditions: Challenges and Perspective.Angewandte Chemie (International ed. in English) · 2026Review
- The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.JACS Au · 2026Review
- Article
- Phase-targeting rapid cryofixation of the beating heart and histological analysis unveil contractile state-dependent sarcomere dynamics.Scientific reports · 2026Article
- Cryogenic light microscopy of vitrified samples with angstrom precision.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Recent advances in Raman probes for multiplexed bioimaging.Inflammation and regeneration · 2025Review
- Engineering Nanomaterials for Raman Bioimaging and Theranostics: Advances, Challenges, and Future Directions.ACS omega · 2025Review
- Time-deterministic cryo-optical microscopy.Light, science & applications · 2025Article
- High-Specificity Spatiotemporal Cholesterol Detection by Quadrature Phase-Shifted Polarization Stimulated Raman Imaging.Angewandte Chemie (International ed. in English) · 2025Article
- Visible enhanced stimulated Raman scattering microscopy via frequency-doubling of picosecond pulses.Biomedical optics express · 2025Article
- Raman imaging as a window into cellular complexity: a future perspective.Nature methods · 2025Article
- Principle of Stimulated Raman Scattering Microscopy: Emerging at High Spatiotemporal Limits.The journal of physical chemistry. C, Nanomaterials and interfaces · 2025Article
- The impact of repeated temperature cycling on cryopreserved human iPSC viability stems from cytochrome redox state changes.Frontiers in bioengineering and biotechnology · 2024Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Raman microscopy is an emerging molecular imaging technology, yet its signal-to-noise ratio (SNR) in measurements of biological specimens is severely limited because of the small cross section of Raman scattering. Here, we present Raman imaging techniques of cryofixed specimens to overcome SNR limitations by enabling long exposure of specimens under highly stabilized low-temperature conditions. The observation of frozen specimens in a cryostat at a constant low temperature immediately after rapid freezing enabled the improvement of SNR and enhanced the spatial and spectral resolution. We also confirmed that the cryofixation can preserve physicochemical states of specimens by observing alkyne-labeled coenzyme Q in cytosol and hemeproteins in acute ischemic myocardium, which cannot be done by fixation using chemical reagents. Last, we applied the technique for multiplex Raman imaging of label-free endogenous molecules and alkyne-tagged molecules in cryofixed HeLa cells, demonstrating its capability of high-content imaging of complex biological phenomena while maintaining physiological conditions.
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