Evidence map›Paper›PMID 39661521›Full record

ArticleCell reports2024

Legionella uses host Rab GTPases and BAP31 to create a unique ER niche.

Attinder Chadha, Yu Yanai, Hiromu Oide, Yuichi Wakana, Hiroki Inoue, Saradindu Saha, Manish Paul, Mitsuo Tagaya, Kohei Arasaki, Shaeri Mukherjee

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Phosphatidic acid production on the vacuole harboringProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Attinder ChadhaG.W. Hooper Foundation, University of California, San Francisco, San Francisco, CA, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.
Yu YanaiSchool of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan.
Hiromu OideSchool of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan.
Yuichi WakanaSchool of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan.
Hiroki InoueSchool of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan.
Saradindu SahaG.W. Hooper Foundation, University of California, San Francisco, San Francisco, CA, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.
Manish PaulG.W. Hooper Foundation, University of California, San Francisco, San Francisco, CA, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.
Mitsuo TagayaSchool of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan.
Kohei ArasakiSchool of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan. Electronic address: karasaki@toyaku.ac.jp.
Shaeri MukherjeeG.W. Hooper Foundation, University of California, San Francisco, San Francisco, CA, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA; Chan Zuckerberg Biohub, San Francisco, CA, USA. Electronic address: shaeri.mukherjee@ucsf.edu.

Funding

Legionella effectors reveal novel signaling function for the ribosome quality control (RQC) pathwayR01GM140440 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MUKHERJEE, SHAERI · 2021 to 2024
$1.7M
Role of HSC70 in protein synthesisR01GM144378 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MUKHERJEE, SHAERI · 2022 to 2025
$1.3M
NIGMS NIH HHS R01 GM140440NIGMS NIH HHS R01 GM144378
6 · The paper itself

Abstract

The bacterium Legionella pneumophila secretes numerous effector proteins that manipulate endoplasmic reticulum (ER)-derived vesicles to form the Legionella-containing vacuole (LCV). Despite extensive studies, whether the LCV membrane is separate from or connected to the host ER network remains unclear. Here, we show that the smooth ER (sER) is closely associated with the LCV early in infection. Remarkably, Legionella forms a distinct rough ER (rER) niche at later stages, disconnected from the host ER network. We discover that host small GTPases Rab10 and Rab4 and an ER protein, BAP31, play crucial roles in transitioning the LCV from an sER to an rER. Additionally, we have identified a Legionella effector, Lpg1152, that binds to BAP31. Interestingly, the optimal growth of Legionella is dependent on both BAP31 and Lpg1152. These findings detail the complex interplay between host and pathogen in transforming the LCV membrane from a host-associated sER to a distinct rER.

Indexed as

Endoplasmic ReticulumLegionella pneumophilarab GTP-Binding ProteinsVacuolesAnimalsBacterial ProteinsHeLa CellsHost-Pathogen InteractionsHumansMembrane ProteinsProtein BindingBacterial ProteinsBCAP31 protein, humanMembrane Proteinsrab GTP-Binding ProteinsbacteriaCP: Cell biologyCP: Microbiologyendoplasmic reticulumLegionellapathogenRab GTPase

Identifiers

PMID39661521
PMCPMC11730038

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.