Evidence map›Paper›PMID 39661504›Full record

ArticleSTAR protocols2024

Protocol for purifying and reconstituting a vacuole membrane transporter Ypq1 into proteoliposomes.

Felichi Mae Arines, Aleksander Wielenga, Randy B Stockbridge, Ming Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Felichi Mae ArinesDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Aleksander WielengaDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Randy B StockbridgeDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Ming LiDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: mlium@umich.edu.

Funding

Structural and functional analysis of novel microbial membrane proteinsR35GM128768 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Randy B. Stockbridge · 2018 to 2026
$3.7M
Characterization of TMEM251 that causes a new type of severe lysosome storage diseaseR01HD109346 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Ming Li · 2022 to 2026
$2.1M
Functional characterization of lysosome membrane and its protein quality control systemR35GM153356 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Ming Li · 2024 to 2026
$1.2M
NICHD NIH HHS R01 HD109346NIGMS NIH HHS R35 GM128768NIGMS NIH HHS R35 GM153356
6 · The paper itself

Abstract

Studying the biochemical function of membrane transporters is important in understanding the biology of transporter-laden organelles such as lysosomes and vacuoles. We present a protocol for overexpressing, purifying, and reconstituting a vacuole membrane transporter Ypq1 into proteoliposomes and describe steps to measure transport activity using radioactive substrates. The protocols established here can be used to study other vacuolar or lysosomal membrane transporters. For complete details on the use and execution of this protocol, please refer to Arines et al.

Indexed as

ProteolipidsVacuolesMembrane Transport ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsMembrane Transport ProteinsProteolipidsproteoliposomesSaccharomyces cerevisiae ProteinsCell MembraneProtein BiochemistryProtein expression and purification

Identifiers

PMID39661504
PMCPMC11697543

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.