Development of a β-lactamase-based aggregation-induced emission lateral flow strip for the detection of clavulanic acid in Milk.

Xiaonan Wang, Tong He, Leina Dou, Licai Ma, Xuezhi Yu, Zhanhui Wang, Kai Wen

Abstract read

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Article in Food chemistry: X, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Xiaonan WangNational Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, and Beijing Laboratory for Food Quality and Safety, Beijing 100193, PR China.

Tong HeNational Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, and Beijing Laboratory for Food Quality and Safety, Beijing 100193, PR China.

Leina DouNational Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, and Beijing Laboratory for Food Quality and Safety, Beijing 100193, PR China.

Licai MaNational Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, and Beijing Laboratory for Food Quality and Safety, Beijing 100193, PR China.

Xuezhi YuNational Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, and Beijing Laboratory for Food Quality and Safety, Beijing 100193, PR China.

Zhanhui WangNational Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, and Beijing Laboratory for Food Quality and Safety, Beijing 100193, PR China.

Kai WenNational Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, and Beijing Laboratory for Food Quality and Safety, Beijing 100193, PR China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lack of biorecognition elements significantly hinders the development of rapid detection methods for clavulanic acid (CA). To address this, we expressed Class A β-lactamases PC1 in vitro and demonstrated its high affinity for CA. Then we investigated the recognition mechanisms of PC1 for CA and identified key contact amino acids: Ser70, Lys73, Ser130, Glu166, and Lys234. Furthermore, PC1 was utilized as a novel biorecognition element to establish a "pseudo-immuno" lateral flow strip (LFS) for CA detection. Aggregation-induced emission fluorescence microspheres (AIE@FM) and biotin-streptavidin (Bio-SA) were integrated to improve the detection performance of PC1-based LFS. Results showed that the sensitivity (cut-off value) of PC1-based AIE(Bio-SA)-LFS was enhanced 2-fold and 4-fold compared to basic AIE@FM-LFS and traditional Au-based LFS, respectively. Eventually, the proposed PC1-based AIE(Bio-SA)-LFS was successfully verified in milk samples with a cut-off value of 20 ng mL

Indexed as

Clavulanic acid;Lateral flow stripPC1 β-lactamasesRapid detection

Identifiers

PMID39659681

DOI10.1016/j.fochx.2024.101950

PMCPMC11629266

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.