Evidence map›Paper›PMID 39658829›Full record

ArticleDiagnostic cytopathology2025

Application of Human Papillomavirus-DNA Test on Salivary Gland Fine Needle Aspiration Cytology Samples Confirms the Absence of the Virus in Primary Neoplasms and Demonstrates for the First Time Its Presence in Salivary Intraglandular Cysts.

Immacolata Cozzolino, Andrea Ronchi, Marco Montella, Raffaella Ruggiero, Rosaria Cappiello, Giovanni Savarese, Giuseppe Colella, Renato Franco

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diagnostic cytopathology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Immacolata CozzolinoPathology Unit, Department of Mental and Physical Health and Preventive Medicine, University of Campania Luigi Vanvitelli, Naples, Italy.ORCID https://orcid.org/0000-0002-6655-0078
Andrea RonchiPathology Unit, Department of Mental and Physical Health and Preventive Medicine, University of Campania Luigi Vanvitelli, Naples, Italy.
Marco MontellaPathology Unit, Department of Mental and Physical Health and Preventive Medicine, University of Campania Luigi Vanvitelli, Naples, Italy.
Raffaella RuggieroAMES-Centro Polidiagnostico Strumentale, SRL, Naples, Italy.
Rosaria CappielloPathology Unit, Department of Mental and Physical Health and Preventive Medicine, University of Campania Luigi Vanvitelli, Naples, Italy.
Giovanni SavareseAMES-Centro Polidiagnostico Strumentale, SRL, Naples, Italy.
Giuseppe ColellaMaxillo-Facial Surgery Unit, Multidisciplinary Department of Medical, Surgical and Dental Specialty, University of Campania Luigi Vanvitelli, Naples, Italy.
Renato FrancoPathology Unit, Department of Mental and Physical Health and Preventive Medicine, University of Campania Luigi Vanvitelli, Naples, Italy.ORCID https://orcid.org/0000-0002-8340-3184

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe correlation between Human Papillomavirus (HPV) and salivary gland neoplasms is still controversial. Data in the literature are conflicting, reporting the presence of the HPV-DNA in a significant percentage of cases or none. We investigated the presence of HPV in a series of salivary gland neoplasms using fine needle aspiration cytology (FNAC) samples to explore its potential oncogenic role in salivary gland tumor development.

methodsThe study included 66 salivary gland lesions from 65 patients. For all cases, residual cytological material in the needle hub, once direct smears were obtained, was fixed by rinsing the needle in Cytolyt hemolytic and preservative solution (Hologic Inc. Marlborough, MA 01752 USA). The samples fixed in the Cytolyt hemolytic and preservative solution (Hologic Inc. Marlborough, MA 01752 USA) were centrifuged at 252 g, then the sediment was transferred to the Preservcyt solution and used for the detection of HPV-DNA.

resultsWe found evidence of the presence of HPV in two salivary intraglandular cysts lined by squamous cell epithelium. Furthermore, we also found HPV in three metastatic oropharyngeal squamous cell carcinomas (SCC) located in the parotid gland. Regarding primary salivary gland tumors, the HPV test produced negative results in all cases.

conclusionHPV testing produced negative results in all primary salivary gland tumors, failing to confirm the potential involvement of HPV infection in the pathogenesis of salivary gland tumors. Instead, the presence of HPV DNA in samples of salivary intraglandular cysts, never reported before, may be an interesting fact, which however requires further evaluation.

Indexed as

CystsDNA, ViralPapillomaviridaePapillomavirus InfectionsSalivary Gland NeoplasmsSalivary GlandsAdultAgedAged, 80 and overBiopsy, Fine-NeedleFemaleHuman Papillomavirus DNA TestsHuman Papillomavirus VirusesHumansMaleMiddle AgedDNA, Viralcystsfine needle aspiration cytologyhuman papillomavirusneoplasmssalivary gland

Identifiers

PMID39658829
PMCPMC11799396

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.