ReviewThe Plant journal : for cell and molecular biology2025
Unlocking regeneration potential: harnessing morphogenic regulators and small peptides for enhanced plant engineering.
Review in The Plant journal : for cell and molecular biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- The glutathione S-transferase gene LaGSTU6 promotes somatic embryogenesis in a recalcitrant lily genotype by regulating scales redox homeostasis.Molecular horticulture · 2026Article
- CRISPR RNP-Mediated Transgene-Free Genome Editing in Plants: Advances, Challenges and Future Directions for Tree Species.Plant, cell & environment · 2026Review
- A versatile chemical toolbox drives plant regeneration.Horticulture research · 2026Article
- Boosting genome editing in perennial plants by CRISPR-Combo mediated morphogenic gene activation.Nature communications · 2026Article
- Plant Regeneration: Influencing Factors, Regulatory Networks, and Epigenetic Mechanisms.International journal of molecular sciences · 2026Review
- Cross-Species Reprogramming of Developmental Plasticity and Metabolic Rewiring via Banana-Derived WUS2 Developmental Regulator.Plant biotechnology journal · 2026Article
- Polyamine-Related Gene Families Identification and Regulatory Effects on Early Somatic Embryogenesis via Modulating Gene Expressions and Hormone Levels inPlants (Basel, Switzerland) · 2026Article
- Establishing SSR-Based Variety Identification and Callus Regeneration Systems for the NovelPlants (Basel, Switzerland) · 2026Article
- Endogenous expression of the wound signal peptide ZmREF1 enhances Agrobacterium-mediated transformation of maize inbred B104.Plant cell reports · 2026Article
- Unravelling the predominant genetic paths for asexual reproduction in Kalanchoe.Nature plants · 2026Article
- Molecular Regulators of In Vitro Regeneration in Wheat: Roles of Morphogenic Factors in Transformation, Genome Editing, and Breeding.International journal of molecular sciences · 2026Review
- Acceleration of flowering in woody plants by grafting.Plant cell reports · 2026Review
- Beyond the Edit: Integrating CRISPR-Cas Genome Editing Into the Cereal Breeding Pipeline.International journal of genomics · 2026Review
- Integrating meristem initiation and floral fate control: towardFrontiers in plant science · 2026Review
- Advances in the use of morphogenic regulators and peptide regenerating factors for boosting plant transformation and genome editing.Frontiers in plant science · 2026Review
- Next-generation CRISPR systems and advanced delivery paradigms for precision crop improvement: a comparative case study in sugar beet (Frontiers in plant science · 2026Review
- Efficient production of gene-edited onion (Allium cepa) plants using biolistic delivery of cas9 RNPs and transient expression constructs.Plant cell reports · 2025Article
- Viral-mediated delivery of morphogenic regulators enables leaf transformation in Sorghum bicolor (L.).Plant biotechnology journal · 2025Article
- From convention to innovation: the role of genetic modification and genome editing in Australian wheat breeding.AoB PLANTS · 2025Review
- Morphogenetic Factors as a Tool for Enhancing Plant Regeneration Capacity During In Vitro Transformation.International journal of molecular sciences · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Plant genetic transformation is essential for understanding gene functions and developing improved crop varieties. Traditional methods, often genotype-dependent, are limited by plants' recalcitrance to gene delivery and low regeneration capacity. To overcome these limitations, new approaches have emerged that greatly improve efficiency and genotype flexibility. This review summarizes key strategies recently developed for plant transformation, focusing on groundbreaking technologies enhancing explant- and genotype flexibility. It covers the use of morphogenic regulators (MRs), stem cell-based methods, and in planta transformation methods. MRs, such as maize Babyboom (BBM) with Wuschel2 (WUS2), and GROWTH-REGULATING FACTORs (GRFs) with their cofactors GRF-interacting factors (GIFs), offer great potential for transforming many monocot species, including major cereal crops. Optimizing BBM/WUS2 expression cassettes has further enabled successful transformation and gene editing using seedling leaves as starting material. This technology lowers the barriers for academic laboratories to adopt monocot transformation systems. For dicot plants, tissue culture-free or in planta transformation methods, with or without the use of MRs, are emerging as more genotype-flexible alternatives to traditional tissue culture-based transformation systems. Additionally, the discovery of the local wound signal peptide Regeneration Factor 1 (REF1) has been shown to enhance transformation efficiency by activating wound-induced regeneration pathways in both monocot and dicot plants. Future research may combine these advances to develop truly genotype-independent transformation methods.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.