ArticleCell reports methods2024
A programmable, open-source robot that scratches cultured tissues to investigate cell migration, healing, and tissue sculpting.
Article in Cell reports methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- The In Vitro Wound-Scratch Assay: Applications, Technical Advances, and Limitations in Wound Healing Research.International wound journal · 2026Review
- In vitro wound simulation: A high-throughput device for scratch assays.Methods (San Diego, Calif.) · 2026Article
- Scratching in style: 3D printers as plotters for automated and complex wound-healing assays.iScience · 2025Article
- Propofol suppresses breast cancer invasion: An in vitro three-dimensional cell invasion model with microfluidic technology.Journal of applied biomedicine · 2025Article
- Comparison of in vitro migration assays evaluating nintedanib's migration inhibitory effects on melanoma cells.Scientific reports · 2025Article
- In Vitro Wound Simulation: A High-Throughput Device for Scratch Assays.bioRxiv : the preprint server for biology · 2025Article
- Dual pathway activation in wound repair: An in vitro study of betanin and theaflavin on periodontal ligament fibroblasts.Journal of oral biology and craniofacial researchArticle
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4 authors.
Funding
Abstract
Despite the widespread popularity of the "scratch assay," where a pipette is dragged manually through cultured tissue to create a gap to study cell migration and healing, it carries significant drawbacks. Its heavy reliance on manual technique can complicate quantification, reduce throughput, and limit the versatility and reproducibility. We present an open-source, low-cost, accessible, robotic scratching platform that addresses all of the core issues. Compatible with nearly all standard cell culture dishes and usable directly in a sterile culture hood without specialized training, our robot makes highly reproducible scratches in a variety of complex cultured tissues with high throughput. Moreover, the robot demonstrates precise removal of tissues for sculpting arbitrary tissue and wound shapes, enabling complex co-culture experiments. This system significantly improves the usefulness of the conventional scratch assay and opens up new possibilities in complex tissue engineering for realistic wound healing and migration research.
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Registered trials
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