Evidence map›Paper›PMID 39656433›Full record

ArticleAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry2025

Photoionization of pyrenemethylamine-labeled oligosaccharides: a new MALDI-TOF precursor ion-type for efficient fragmentation.

Andreas H Franz, Nathan Ta, Ella Nguyen, Kendall Bromley, Hana Rosenblatt, Aprilrose Fabro, Geoff Lin-Cereghino

Abstract read
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In one paragraph

Article in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Andreas H FranzDepartment of Chemistry, University of the Pacific, 3601 Pacific Avenue, Stockton, CA, 95211, USA. afranz@pacific.edu.ORCID http://orcid.org/0000-0002-3016-516X
Nathan TaDepartment of Chemistry, University of the Pacific, 3601 Pacific Avenue, Stockton, CA, 95211, USA.
Ella NguyenDepartment of Chemistry, University of the Pacific, 3601 Pacific Avenue, Stockton, CA, 95211, USA.
Kendall BromleyDepartment of Chemistry, University of the Pacific, 3601 Pacific Avenue, Stockton, CA, 95211, USA.
Hana RosenblattDepartment of Chemistry, University of the Pacific, 3601 Pacific Avenue, Stockton, CA, 95211, USA.
Aprilrose FabroDepartment of Chemistry, University of the Pacific, 3601 Pacific Avenue, Stockton, CA, 95211, USA.
Geoff Lin-CereghinoDepartment of Biology, University of the Pacific, 3601 Pacific Avenue, Stockton, CA, 95211, USA.

Funding

National Institute of Health 1 R15 GM129758-01
6 · The paper itself

Abstract

Oligosaccharides were covalently labeled with 1-pyrenemethylamine (1-PMA) in 15 min at 75 °C with high yield, separated by quantitative HPLC in less than 20 min, and characterized by off-line Matrix-Assisted Laser Desorption/Ionization (MALDI) Time-of-Flight (TOF) mass spectrometry (MS). A new MALDI mass spectral precursor ion for fragmentation studies was observed from 2,5-dihydroxybenzoic acid matrix (DHB, hydroquinone/benzoquinone redox system) through photo-induced reductive elimination. The resulting high-energy protonated glycosylamine provided excellent fragmentation efficiency (Y-, B-ions, and combination losses), with ions covering almost the entire structure of several oligosaccharides at the low picomol level. With the new method, glycans from commercially available standard glycoproteins and glycans from bioengineered β-lactoglobulin expressed in the bgs13 mutant of Pichia pastoris were analyzed.

Indexed as

OligosaccharidesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationIonsPhotochemical ProcessesIonsOligosaccharidesHPLCMALDI-TOF mass spectrometryOligosaccharide labelingPyrenemethylamineUV-active glycans

Identifiers

PMID39656433

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.