ArticleThe Journal of cell biology1985
Identical distribution of fluorescently labeled brain and muscle actins in living cardiac fibroblasts and myocytes.
Article in The Journal of cell biology, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 78 citations in OpenAlex.
- Mechanosignaling pathways alter muscle structure and function by post-translational modification of existing sarcomeric proteins to optimize energy usage.Journal of muscle research and cell motility · 2021Article
- CapZ and actin capping dynamics increase in myocytes after a bout of exercise and abates in hours after stimulation ends.Journal of applied physiology (Bethesda, Md. : 1985) · 2013Article
- Titin visualization in real time reveals an unexpected level of mobility within and between sarcomeres.The Journal of cell biology · 2011Article
- Dynamic regulation of sarcomeric actin filaments in striated muscle.Cytoskeleton (Hoboken, N.J.) · 2010Review
- Context-dependent functional substitution of alpha-skeletal actin by gamma-cytoplasmic actin.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- Properties of easily releasable myofilaments: are they the first step in myofibrillar protein turnover?American journal of physiology. Cell physiology · 2009Article
- Differential effects of Latrunculin-A on myofibrils in cultures of skeletal muscle cells: insights into mechanisms of myofibrillogenesis.Cell motility and the cytoskeleton · 2005Article
- Dynamics of Z-band based proteins in developing skeletal muscle cells.Cell motility and the cytoskeleton · 2005Article
- The specific NH2-terminal sequence Ac-EEED of alpha-smooth muscle actin plays a role in polymerization in vitro and in vivo.The Journal of cell biology · 1995Article
- Mechanisms of thin filament assembly in embryonic chick cardiac myocytes: tropomodulin requires tropomyosin for assembly.The Journal of cell biology · 1995Article
- The binding of fluorescent phallotoxins to actin in myofibrils.Journal of muscle research and cell motility · 1993Article
- Isoform-specific 3'-untranslated sequences sort alpha-cardiac and beta-cytoplasmic actin messenger RNAs to different cytoplasmic compartments.The Journal of cell biology · 1993Article
- Beta and gamma actin mRNAs are differentially located within myoblasts.The Journal of cell biology · 1993Article
- Incorporation of microinjected biotin-labelled actin into nascent myofibrils of cardiac myocytes: an immunoelectron microscopic study.Journal of muscle research and cell motility · 1993Article
- Molecular genetics of actin function.The Biochemical journal · 1993Review
- In vitro and in vivo characterization of four fibroblast tropomyosins produced in bacteria: TM-2, TM-3, TM-5a, and TM-5b are co-localized in interphase fibroblasts.The Journal of cell biology · 1992Article
- High level expression of transfected beta- and gamma-actin genes differentially impacts on myoblast cytoarchitecture.The Journal of cell biology · 1992Article
- Chicken cardiac myofibrillogenesis studied with antibodies specific for titin and the muscle and nonmuscle isoforms of actin and tropomyosin.Cell and tissue research · 1991Article
- Functional sorting of actin isoforms in microvascular pericytes.The Journal of cell biology · 1989Article
- Functional alterations in beta'-actin from a KB cell mutant resistant to cytochalasin B.The Journal of cell biology · 1988Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated whether living muscle and nonmuscle cells can discriminate between microinjected muscle and nonmuscle actins. Muscle actin purified from rabbit back and leg muscles and labeled with fluorescein isothiocyanate, and nonmuscle actin purified from lamb brain and labeled with lissamine rhodamine B sulfonyl chloride, were co-injected into chick embryonic cardiac myocytes and fibroblasts. When fluorescence images of the two actins were compared using filter sets selective for either fluorescein isothiocyanate or lissamine rhodamine B sulfonyl chloride, essentially identical patterns of distribution were detected in both muscle and nonmuscle cells. In particular, we found no structure that, at this level of resolution, shows preferential binding of muscle or nonmuscle actin. In fibroblasts, both actins are associated primarily with stress fibers and ruffles. In myocytes, both actins are localized in sarcomeres. In addition, the distribution of structures containing microinjected actins is similar to that of structure containing endogenous F-actin, as revealed by staining with fluorescent phalloidin or phallacidin. Our results suggest that, at least under these experimental conditions, actin-binding sites in muscle and nonmuscle cells do not discriminate among different forms of actins.
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