Physical and Chemical Characterization of Aerosols Produced from Commercial Nicotine Salt-Based E-Liquids.

Nicotine salt e-liquids are widely used in pod-style and disposable electronic nicotine delivery systems (ENDS). Studying the physical and chemical properties of their emissions can inform their toxicological impact. A prior companion study reported the harmful and potentially harmful constituents (HPHCs) and aerosol particle sizes produced from laboratory-made nicotine salt and freebase nicotine e-liquids to assess the effects of varying nicotine salts and nicotine protonation. This study reports the HPHCs and aerosol particle sizes for commercial brand nicotine salt and freebase nicotine formulations. Several tobacco, fruit, mint, and menthol flavored e-liquids of varying nicotine concentrations were tested with open and closed pod-style ENDS and a disposable ENDS. The nicotine yields showed a positive correlation with aerosol output, and the aerosol nicotine mass fractions reflected the e-liquid nicotine quantities. Benzene, crotonaldehyde, and 2,3-pentanedione were not detected or quantified in any of the aerosols, whereas acetaldehyde, acrolein, diacetyl, and formaldehyde were each quantified in at least one of the tested conditions. The aerosol particle number concentrations indicated that 97-99% of the aerosols for all the ENDS tested were composed of ultrafine (<0.1 μm) and fine (0.1-1.0 μm) aerosol particle sizes, and the mass median aerodynamic diameters ranged from 1.0 to 1.4 μm. The estimated regional deposition fractions and total respiratory depositions were calculated for all the ENDS conditions using a dosimetry modeling program. The calculations predicted depositions would predominantly occur in the pulmonary and head regions with a low total respiratory deposition (≤41%) calculated for all ENDS tested. This study broadens the availability of high-quality and reliable testing data of popular commercial nicotine salt-based ENDS for the scientific and regulatory communities. In conjunction with the previous work on the model e-liquids, these studies offer an extensive examination of the HPHCs and physical aerosol parameters of nicotine salt e-liquids.