ArticleCurrent microbiology2024
Transcriptome Sequencing of Bacillus sp. TTMP20 and Analysis of the Effect of BDH Gene on Tetramethylpyrazine Synthesis.
Shiyu Liang, Mengxiang Ling, Lihua Li, Shanling Gan, Changhua Shang
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In one paragraphArticle in Current microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 authors.
Shiyu LiangKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China.
Mengxiang LingKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China.
Lihua LiKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China.
Shanling GanKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China. ganshanling1999@163.com.
Changhua ShangKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China. shangchanghua@mailbox.gxnu.edu.cn.ORCID http://orcid.org/0000-0003-1447-6117 Funding
Guangxi Key Research and Development Program 2021AB27009Guangxi Key Research and Development Program 2023AB01132
6 · The paper itselfAbstract
Bacillus sp. TTMP20 has high tetramethylpyrazine (TTMP) yield. However, the mechanism of TTMP production in this strain is unclear at present, which limits the modification for strain TTMP20. In this study, key metabolic pathways related to TTMP synthesis were identified, which included glycolytic pathway, tricarboxylic acid cycle and nitrogen metabolism pathway. Moreover, transgenic Bacillus sp. TTMP20 with recombinant vector PHY-300PLK-BDH was constructed. 2,3-butanediol dehydrogenase gene (BDH) overexpression considerably reduced TTMP biosynthesis. This study will supply new insight into the regulation of TTMP biosynthesis at key enzyme gene level.
Indexed as
Alcohol OxidoreductasesBacillusPyrazinesBacterial ProteinsGene Expression Regulation, BacterialMetabolic Networks and PathwaysTranscriptomeAlcohol OxidoreductasesBacterial Proteinsbutanediol dehydrogenasePyrazinestetramethylpyrazine
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