Evidence map›Paper›PMID 39652549›Full record

ArticlePloS one2024

Enhancing reproducibility in single cell research with biocytometry: An inter-laboratory study.

Pavel Fikar, Laura Alvarez, Laura Berne, Martin Cienciala, Christopher Kan, Hynek Kasl, Mona Luo, Zuzana Novackova, Sheyla Ordonez, Zuzana Sramkova and 41 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

51 authors.

Pavel FikarDepartment of Research & Development, Sampling Human Inc., Berkeley, California, United States of America.ORCID 0000-0002-5888-7551
Laura AlvarezDepartment of Research & Development, Sampling Human Inc., Berkeley, California, United States of America.
Laura BerneDepartment of Research & Development, Sampling Human Inc., Berkeley, California, United States of America.
Martin CiencialaDepartment of Research & Development, Sampling Human Inc., Berkeley, California, United States of America.
Christopher KanDepartment of Research & Development, Sampling Human Inc., Berkeley, California, United States of America.ORCID 0000-0002-2265-131X
Hynek KaslDepartment of Research & Development, Sampling Human Inc., Berkeley, California, United States of America.
Mona LuoDepartment of Research & Development, Sampling Human Inc., Berkeley, California, United States of America.
Zuzana NovackovaDepartment of Research & Development, Sampling Human Inc., Berkeley, California, United States of America.
Sheyla OrdonezDepartment of Research & Development, Sampling Human Inc., Berkeley, California, United States of America.
Zuzana SramkovaDepartment of Research & Development, Sampling Human Inc., Berkeley, California, United States of America.
Monika HolubovaDepartment of Hematology and Oncology, University Hospital Pilsen, Pilsen, Czech Republic.
Daniel LysakDepartment of Hematology and Oncology, University Hospital Pilsen, Pilsen, Czech Republic.
Lyndsay AveryDepartment of Biology, Saint Michael's College, Colchester, Vermont, United States of America.
Andres A CaroChemistry Department, Hendrix College, Conway, Arkansas, United States of America.
Roslyn N CrowderDepartment of Biology, Stetson University, Deland, Florida, United States of America.
Laura A Diaz-MartinezDepartment of Biology, Gonzaga University, Spokane, Washington, United States of America.ORCID 0000-0002-7226-7068
David W DonleyDepartment of Biology, Harding University, Searcy, Arkansas, United States of America.ORCID 0000-0001-8939-981X
Rebecca R GiornoSchool of Biological Sciences, Louisiana Tech University, Ruston, Louisiana, United States of America.
Irene K Guttilla ReedDepartment of Biology, University of Saint Joseph, West Hartford, Connecticut, United States of America.ORCID 0000-0002-6617-1465
Lori L HensleyDepartment of Biological Sciences, Jacksonville State University, Jacksonville, Alabama, United States of America.
Kristen C JohnsonDepartment of Life Sciences, University of New Hampshire, Manchester, New Hampshire, United States of America.ORCID 0000-0002-1825-0265
Audrey Y KimDepartment of Biological Sciences, Grambling State University, Grambling, Louisiana, United States of America.
Paul KimDepartment of Biological Sciences, Grambling State University, Grambling, Louisiana, United States of America.ORCID 0000-0003-4936-0261
Adriana J LaGierDepartment of Biology, College of Social and Natural Sciences, Grand View University, Des Moines, Iowa, United States of America.ORCID 0000-0002-6135-1424
Jamie J NewmanSchool of Biological Sciences, Louisiana Tech University, Ruston, Louisiana, United States of America.ORCID 0000-0002-1537-350X
Elizabeth Padilla-CrespoDepartment of Science and Technology, Inter American University of Puerto Rico-Aguadilla, Aguadilla, Puerto Rico, United States of America.ORCID 0000-0002-1038-3987
Nathan S ReynaDepartment of Biology, Ouachita Baptist University, Arkadelphia, Arkansas, United States of America.ORCID 0000-0003-3430-0282
Nikolaos TsotakosDepartment of Biological Sciences, School of Science Engineering and Technology, Penn State Harrisburg, Middletown, Pennsylvania, United States of America.ORCID 0000-0002-1028-3062
Noha N Al-SaadiDepartment of Biological Sciences, Jacksonville State University, Jacksonville, Alabama, United States of America.
Tayler AppletonDepartment of Biology, Harding University, Searcy, Arkansas, United States of America.
Ana Arosemena-PickettDepartment of Science and Technology, Inter American University of Puerto Rico-Aguadilla, Aguadilla, Puerto Rico, United States of America.
Braden A BellDepartment of Biology, Gonzaga University, Spokane, Washington, United States of America.
Grace BingDepartment of Biology, Harding University, Searcy, Arkansas, United States of America.
Bre BishopDepartment of Biology, Harding University, Searcy, Arkansas, United States of America.
Christa FordeDepartment of Biological Sciences, Grambling State University, Grambling, Louisiana, United States of America.
Michael J FosterSchool of Biological Sciences, Louisiana Tech University, Ruston, Louisiana, United States of America.ORCID 0000-0002-6006-0641
Kassidy GrayDepartment of Biology, Ouachita Baptist University, Arkadelphia, Arkansas, United States of America.
Bennett L HasleyDepartment of Biological Sciences, Jacksonville State University, Jacksonville, Alabama, United States of America.
Kennedy JohnsonDepartment of Biology, Ouachita Baptist University, Arkadelphia, Arkansas, United States of America.
Destiny J JonesDepartment of Biological Sciences, Grambling State University, Grambling, Louisiana, United States of America.
Allison C LaShallDepartment of Biological Sciences, Jacksonville State University, Jacksonville, Alabama, United States of America.
Kennedy McGuireDepartment of Biology, Ouachita Baptist University, Arkadelphia, Arkansas, United States of America.
Naomi McNaughtonDepartment of Biology, Harding University, Searcy, Arkansas, United States of America.
Angelina M MorganDepartment of Biological Sciences, Grambling State University, Grambling, Louisiana, United States of America.
Lucas NorrisSchool of Biological Sciences, Louisiana Tech University, Ruston, Louisiana, United States of America.
Landon A OssmanSchool of Biological Sciences, Louisiana Tech University, Ruston, Louisiana, United States of America.
Paollette A Rivera-TorresDepartment of Science and Technology, Inter American University of Puerto Rico-Aguadilla, Aguadilla, Puerto Rico, United States of America.
Madeline E RobisonSchool of Biological Sciences, Louisiana Tech University, Ruston, Louisiana, United States of America.ORCID 0009-0002-1994-3917
Kathryn ThibodauxSchool of Biological Sciences, Louisiana Tech University, Ruston, Louisiana, United States of America.
Lescia ValmondDepartment of Biological Sciences, Grambling State University, Grambling, Louisiana, United States of America.
Daniel GeorgievDepartment of Research & Development, Sampling Human Inc., Berkeley, California, United States of America.ORCID 0009-0008-2165-8561

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Biomedicine today is experiencing a shift towards decentralized data collection, which promises enhanced reproducibility and collaboration across diverse laboratory environments. This inter-laboratory study evaluates the performance of biocytometry, a method utilizing engineered bioparticles for enumerating cells based on their surface antigen patterns. In centralized and aggregated inter-lab studies, biocytometry demonstrated significant statistical power in discriminating numbers of target cells at varying concentrations as low as 1 cell per 100,000 background cells. User skill levels varied from expert to beginner capturing a range of proficiencies. Measurement was performed in a decentralized environment without any instrument cross-calibration or advanced user training outside of a basic instruction manual. The results affirm biocytometry to be a viable solution for immunophenotyping applications demanding sensitivity as well as scalability and reproducibility and paves the way for decentralized analysis of rare cells in heterogeneous samples.

Indexed as

Single-Cell AnalysisFlow CytometryHumansImmunophenotypingLaboratoriesReproducibility of Results

Identifiers

PMID39652549
PMCPMC11627387

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.