An efficient method for visualizing the plaques of Bdellovibrio bacteriovorus.

Qian Zhao, Jiangong Xu, Meirong Song, Kui Zhu

Abstract read

In one paragraph

Article in Archives of microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Research Advances on the Impact of Gut Microbiota on COPD: Exploring New Perspectives on the Microbiota-Gut-Lung Axis.Current microbiology · 2025
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Qian ZhaoNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.

Jiangong XuTechnology Innovation Center for Food Safety Surveillance and Detection (Hainan), Sanya Institute of China Agricultural University, Sanya, 572025, China.

Meirong SongNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.

Kui ZhuNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China. zhuk@cau.edu.cn.

Funding

National Key Research and Development Program of China 2022YFD1801600National Natural Science Foundation of China 32230106

6 · The paper itself

Abstract

Bdellovibrio bacteriovorus, an obligate predator of Gram-negative bacteria, has emerged as a promising natural antibiotic to combat the escalating threat of antibiotic resistance. Plaque forming units (PFU) counting is commonly used to determine the viable numbers of B. bacteriovorus. However, nearly three-days incubation is always necessary for getting the single, obvious plaques on the double-layer agar plate. This time-consuming procedure greatly impedes the purification and enumeration efficiency of B. bacteriovorus. Until now, no simplified method has been developed to address this issue. In this work, we evaluated the advantages of using fluorescent prey to enhance the visualization of the B. bacteriovorus plaques. Our work reveals that the regular single plaques are easily observed under the enhanced background of fluorescent prey lawn in one and a half days, reducing nearly half of the time consumption in the purification and enumeration of B. bacteriovorus.

Indexed as

Bdellovibrio bacteriovorusBdellovibrio bacteriovorusDouble-layer agar plateFluorescent preyPlaquesVisualization

Identifiers

PMID39652181

DOI10.1016/j.tim.2023.10.005

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.