ArticleJDS communications2024
Assessment of blood sampling time points to determine the relative bioavailability of ruminally protected methionine supplements using the plasma free amino acid dose-response technique.
Article in JDS communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Bioavailability of rumen-protected L-methionine supplement in lactating dairy cows.JDS communications · 2026Article
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Authors and funding
6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The calculation of the relative bioavailability (RBV) of rumen-protected AA supplements using the plasma free AA dose-response technique currently relies on blood samples obtained 2, 4, 6, and 8 h after the 0500 h feeding during the last 3 d of each period in Latin square experiments with cows fed every 8 h (0500, 1300, and 2100 h). The objective of this study was to determine if this current blood sampling protocol captures the changes that may occur in plasma Met concentrations within a 24-h day to adequately determine the RBV of Met from Smartamine M (SM). Five multiparous lactating Holstein cows were used in a 5 × 5 Latin square design with 7-d periods. Treatments were (1) control (abomasal infusion of tap water), (2) 12 g/d of abomasally infused dl-Met, (3) 24 g/d of abomasally infused dl-Met, (4) 15 g/d of fed Met (20 g/d of SM), and (5) 30 g/d of fed Met (40 g/d of SM). Blood samples were collected via jugular catheters every 2 h after the 0500 h feeding starting on d 5 and ending on d 7 of each period. Plasma Met analysis was conducted using gas chromatography after chloroformate derivatization. Plasma Met concentration was averaged across days for 2-8 h after the 0500 h feeding, 2-8 h after the 1300 h feeding, 2-8 h after the 2100 h feeding, and 2-24 h after the 0500 h feeding. In addition, plasma Met concentration was regressed on 0, 12, and 24 g of infused dl-Met and 0, 15, and 30 g of fed Met. The calculated RBV of Met from SM averaged 83.8%, 83.6%, 87.4%, and 83.0% for the 2-8 h, 10-16 h, 18-24 h, and 2-24 h sampling periods, respectively. The similarity in the estimations of RBV for the 2-8 h and 2-24 h sampling periods indicates that our original blood sampling protocol seems reliable for determining the RBV of ruminally protected Met products.
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