ArticlePlant biotechnology journal2025
An R2R3-type MYB transcription factor, GmMYB77, negatively regulates isoflavone accumulation in soybean [Glycine max (L.) Merr.].
Article in Plant biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Integrated transcriptomic and metabolomic analysis unveils biosynthetic mechanisms of key bioactive isoflavonoid accumulation inHorticulture research · 2026Article
- Article
- Comparative Metabolomics and Network Pharmacology Study Reveals Chemopreventive Potential of Wild Soybean (Foods (Basel, Switzerland) · 2026Article
- Phosphorus-driven rhizobial community assembly underpins superior nitrogen fixation efficiency in high-oil soybean.BMC plant biology · 2026Article
- Leveraging weighted embedding and Transformer architecture to improve phenotype prediction of complex traits for crops.Nature communications · 2026Article
- GmMYB4 Positively Regulates Isoflavone Biosynthesis via the GmMAPK6-GmMYB4-MBW Module in Soybean.Plant biotechnology journal · 2026Article
- A balance between glycitein and glyceollins governed by isoflavone 6-hydroxylase confers soybean resistance toProceedings of the National Academy of Sciences of the United States of America · 2025Article
- An MYB transcription factor regulates capsaicinoid biosynthesis during pepper fruit ripening.BMC plant biology · 2025Article
- A R2R3-MYB Transcription Factor ofInternational journal of molecular sciences · 2025Article
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10 authors.
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Abstract
Soybean [Glycine max (L.) Merr.] is an exceptionally rich in isoflavones, and these compounds attach to oestrogen receptors in the human body, lessening the risk of breast cancer and effectively alleviating menopausal syndrome symptoms. Uncovering the molecular mechanisms that regulate soybean isoflavone accumulation is crucial for enhancing the production of these compounds. In this study, we combined bulk segregant analysis sequencing (BSA-seq) and a genome-wide association study (GWAS) to discover a novel R2R3-MYB family gene, GmMYB77, that regulates isoflavone accumulation in soybean. Using the soybean hairy root transient expression system, we verified that GmMYB77 inhibits isoflavone accumulation. Furthermore, knocking out GmMYB77 significantly increased total isoflavone (TIF) content, particularly malonylglycitin, while its overexpression resulted in a notable decrease in contents of malonylglycitin and TIF. We found that GmMYB77 can directly binds the core sequence GGT and suppresses the expression of the key isoflavone biosynthesis genes Isoflavone synthase 1 (GmIFS1), Isoflavone synthase 2 (GmIFS2), Chalcone synthase 7 (GmCHS7) and Chalcone synthase 8 (GmCHS8) by using dual-luciferase assays, electrophoretic mobility shift assays and yeast one-hybrid experiments. Natural variations in the promoter region of GmMYB77 affect its expression, thereby regulating the malonylglycitin and TIF contents. Hap-P2, an elite haplotype, plays a pivotal role in soybean breeding for substantially enhanced isoflavone content. These findings enhance our understanding of the genes influencing soybean isoflavone content and provide a valuable genetic resource for molecular breeding efforts in the future.
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