Evidence map›Paper›PMID 39645655›Full record

ArticleSTAR protocols2024

Protocol for measuring anti-fentanyl antibodies in mouse serum by enzyme-linked immunosorbent assay.

Jacob A Koster, Alejandra Prevost-Reilly, Dheeraj Soni, Aisling Kelly, Kristina Walker, Elizabeth B Norton, Ofer Levy, Colin N Haile, David J Dowling

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jacob A KosterPrecision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA.
Alejandra Prevost-ReillyPrecision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA.
Dheeraj SoniPrecision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA; Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA; Global Investigative Toxicology, Preclinical Safety, Sanofi, Cambridge, MA, USA.
Aisling KellyPrecision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA.
Kristina WalkerPrecision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA.
Elizabeth B NortonDepartment of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA 70112, USA.
Ofer LevyPrecision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA; Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA; Broad Institute of MIT & Harvard, Cambridge, MA 02142, USA.
Colin N HaileDepartment of Psychology/TIMES, University of Houston, Houston, TX 77204, USA.
David J DowlingPrecision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA; Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA. Electronic address: david.dowling@childrens.harvard.edu.

Funding

Development of Novel Adjuvants LTA and LTA1R01AI114697 · NIAID · TULANE UNIVERSITY OF LOUISIANA · PI NORTON, ELIZABETH B · 2015 to 2019
$2.1M
NIAID NIH HHS 75N93020C00038NIAID NIH HHS R01 AI114697
6 · The paper itself

Abstract

Enzyme-linked immunosorbent assay (ELISA) offers an effective, inexpensive, and reliable approach for the analysis of humoral immune responses. Here, we describe a protocol for measuring anti-fentanyl antibodies generated by the immunization of mice with novel opioid vaccine candidates. We describe steps for coating BSA-fentanyl antigen and standard wells. We then detail procedures for preparing buffers and performing assays. This protocol is adaptable for use with either BALB/c or C57BL/6J mice strains and can measure antibody isotypes to elucidate class switching. For complete details on the use and execution of this protocol, please refer to Stone et al.

Indexed as

AntibodiesEnzyme-Linked Immunosorbent AssayFentanylMice, Inbred BALB CMice, Inbred C57BLAnimalsMiceAntibodiesFentanylantibodycell biologyimmunology

Identifiers

PMID39645655
PMCPMC11667700

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.