Evidence map›Paper›PMID 39644497›Full record

ArticleSTAR protocols2024

Protocol to measure glucose utilization in mouse tissues using radiolabeled 2-deoxy-D-glucose.

Cyun-Ming Wu, Hao-Yun Li, Wen-Yi Li, Chih-Neng Hsu, Wei-Shun Yang, Gwo-Tsang Chuang, Tony Pan-Hou Che, Tung-Yuan Lee, Hsiao-Lin Lee, Siow-Wey Hee and 7 more

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Article in STAR protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Cyun-Ming WuDepartment of Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan.
Hao-Yun LiDepartment of Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan.
Wen-Yi LiDivision of Nephrology, Department of Internal Medicine, National Taiwan University Hospital, Yunlin Branch, Yunlin 640, Taiwan.
Chih-Neng HsuCardiovascular Center, National Taiwan University Hospital, Yunlin Branch, Yunlin 640, Taiwan.
Wei-Shun YangDivision of Nephrology, Department of Internal Medicine, National Taiwan University Hospital, HsinChu Branch, HsinChu 300, Taiwan.
Gwo-Tsang ChuangDivision of Nephrology, Department of Pediatrics, National Taiwan University Hospital, Taipei 100, Taiwan.
Tony Pan-Hou ChePhD Program in Translational Medicine, National Taiwan University and Academia Sinica, Taipei 100, Taiwan.
Tung-Yuan LeePhD Program in Translational Medicine, National Taiwan University and Academia Sinica, Taipei 100, Taiwan.
Hsiao-Lin LeeDepartment of Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan; Graduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, Taiwan.
Siow-Wey HeeDivision of Endocrinology and Metabolism, Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan.
Jiin-Horng LeeGraduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, Taiwan.
Daniel LiaoDivision of Endocrinology and Metabolism, Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan.
Karen Chia-Wen LiaoBiological Sciences Division, University of Chicago, Chicago, IL 60637, USA.
Ya-Wen LiuInstitute of Molecular Medicine, National Taiwan University, Taipei 100, Taiwan.
Chin-Feng HsuanDivision of Cardiology, Department of Internal Medicine, E-Da Hospital, I-Shou University, Kaohsiung 824, Taiwan; Division of Cardiology, Department of Internal Medicine, E-Da Dachang Hospital, I-Shou University, Kaohsiung 807, Taiwan; School of Medicine, College of Medicine, I-Shou University, Kaohsiung 824, Taiwan.
Yi-Cheng ChangPhD Program in Translational Medicine, National Taiwan University and Academia Sinica, Taipei 100, Taiwan; Division of Endocrinology and Metabolism, Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan; Graduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, Taiwan; Institute of Biomedical Sciences, Academia Sinica, Taiwan 100, Taiwan. Electronic address: yichengchang@ntu.edu.tw.
Shao-Lun ChuDepartment of Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan. Electronic address: dslchu@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

2-deoxy-D-glucose (2DG) is a glucose analog converted to 2-deoxy-D-glucose-6-phosphate (2DG-6P) by hexokinase in glycolysis. While 2DG commonly measures glucose uptake, 2DG-6P detects glucose utilization. Here, we present a protocol to measure glucose utilization in various tissues after entering a mouse's body using radiolabeled 2DG. We describe steps for preparing mice and chemicals, extracting blood, adding chemicals, and dissolving tissue. We then detail procedures for calculating glucose utilization using the trapezoid rule.

Indexed as

DeoxyglucoseGlucoseAnimalsGlucose-6-PhosphateMice2-deoxyglucose-6-phosphateDeoxyglucoseGlucoseGlucose-6-PhosphateHealth SciencesMetabolismMetabolomics

Identifiers

PMID39644497
PMCPMC11656071

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.