Evidence map›Paper›PMID 39644360›Full record

ArticleMolecular biology reports2024

Transcription factor E93 regulates vitellogenesis via the vitelline membrane protein 26Ab gene in Chilo Suppressalis.

Siyang Chen, Yingjuan Sun, Suijie Kuang, Yan Tang, Wenbing Ding, Hualiang He, Jin Xue, Qiao Gao, Hongshuai Gao, Youzhi Li and 1 more

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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2 citing papers in PubMed.

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5 · Who and what money

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11 authors.

Siyang Chen *Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China.
Yingjuan Sun *Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China.
Suijie KuangHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China.
Yan TangHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China.
Wenbing DingNational Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China.
Hualiang HeHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China.
Jin XueHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China.
Qiao GaoHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China.
Hongshuai GaoNational Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China.
Youzhi LiHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China.
Lin QiuHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China. qiulin@hunau.edu.cn.

Funding

Hunan graduate Research Innovation Project Grant No. CX20220690Hunan Provincial Natural Science Foundation of China 2022JJ30300National Natural Science Foundation of China 31801736National Natural Science Foundation of China 32472557science and technology innovation Program of Hunan Province 2022RC1025
6 · The paper itself

Abstract

backgroundEcdysone-induced protein 93 F (E93, also known as Eip93F) plays a crucial role in the reproductive process of numerous insects. This study aims to delineate the function of E93 in Chilo suppressalis and elucidated the regulatory mechanism by which E93 influences the reproduction of C. suppressalis METHODS AND

resultsThe results of the bioinformatics analysis indicate that C. suppressalis E93 shows the highest homology with E93 from Bombyx mori. We used qPCR to evaluate the expression profile of CsE93 from different developmental stages and tissues, revealed that CsE93 had the highest expression levels in the head, which peaked during the prepupal stage. Silencing CsE93 resulted in a significant reduction in yolk deposition and abnormal ovarian development. Moreover, the transcriptional levels of vitellogenin (Vg) and E74A, which are related to vitellogenesis and the 20E pathway, were significantly down-regulated in dsE93-treated female pupae. In addition, we identified Vitelline membrane protein 26Ab (VMP26Ab), a downstream gene associated with the integrity of the inner eggshell. The knockdown of VMP26Ab resulted in a significant reduction in the number of eggs and abnormal ovarian development, similar to RNAi E93. Finally, we identified an active promoter fragment (containing GAGA-containing motif) of CsVMP26Ab and demonstrated that CsE93 can bind to it.

resultsOur results indicate that CsE93 plays an important role in C. suppressalis reproduction. CsE93 modulates the CsVMP26Ab expression by acting on its promoter involve in the reproduction of C. suppressalis finally.

Indexed as

Insect ProteinsVitellogenesisVitellogeninsAnimalsEgg ProteinsFemaleGene Expression Regulation, DevelopmentalMembrane ProteinsMothsOvaryPromoter Regions, GeneticTranscription FactorsEgg ProteinsInsect ProteinsMembrane ProteinsTranscription Factorsvitelline membrane proteinsVitellogeninsChilo SuppressalisEcdysone-induced protein 93FReproductionVitelline membrane protein 26Ab

Identifiers

PMID39644360

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.