Evidence map›Paper›PMID 39643758›Full record

ArticleCellular and molecular life sciences : CMLS2024

BRG1 improves reprogramming efficiency by enhancing glycolytic metabolism.

Xuan Ren, Shihai Huang, Jianchun Xu, Qingsong Xue, Tairan Xu, Deshun Shi, Shinan Ma, Xiangping Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xuan RenGuangxi Key Laboratory of Animal Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning, 530005, China.
Shihai HuangCollege of Life Science and Technology, Guangxi University, Nanning, 530005, China.
Jianchun XuGuangxi Key Laboratory of Animal Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning, 530005, China.
Qingsong XueGuangxi Key Laboratory of Animal Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning, 530005, China.
Tairan XuGuangxi Key Laboratory of Animal Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning, 530005, China.
Deshun ShiGuangxi Key Laboratory of Animal Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning, 530005, China.
Shinan MaHubei Key Laboratory of Embryonic Stem Cell Research, Hubei Provincial Clinical Research Center for Umbilical Cord Blood Hematopoietic Stem Cells, Tai-He Hospital, Hubei University of Medicine, Shiyan, 442000, Hubei, China. mashinan2021@163.com.
Xiangping LiGuangxi Key Laboratory of Animal Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning, 530005, China. xiangpingli@163.com.ORCID http://orcid.org/0000-0002-2560-3065

Funding

Guangxi Natural Science Funding 2020GXNSFDA297009National Natural Science Funding 32060754National Natural Science Funding 32460833Natural Science Foundation of Hubei Province 2024AFB787Project of Bama County for Talents in Science and Technology 20220030
6 · The paper itself

Abstract

BRG1 has been found to promote the generation of induced pluripotent stem cells (iPSCs) by regulating epigenetic modifications or binding to transcription factors, however, the role of BRG1 on the cellular metabolism during reprogramming has not been reported. In this study, we found that BRG1 improved the efficiency of porcine iPSC generation, and upregulated the expression of pluripotency-related factors. Further analysis revealed that BRG1 promoted cellular glycolysis, and increased levels of glycolysis-related metabolites. It enhanced the transcriptional activity of glycolysis-related gene HK2, PKM2, and PFK-1 promoters, and decreased the enrichment of H3K9me3 in glycolysis- and pluripotency-related gene promoters. BRG1 also increased the phosphorylation level at the Ser473 site of AKT protein. The specific PI3K/AKT signaling pathway inhibitor, LY294002, impaired the generation of porcine iPSCs, downregulated the expression of pluripotency-related factors, and inhibited cellular glycolysis, overexpressing BRG1 rescued those changes caused by LY294002 treatment. In addition, the glycolysis inhibitor 2-DG and BRG1 inhibitor PFI-3 had similar effects to LY294002. The above results suggest that overexpression of BRG1 promotes the generation of porcine iPSCs by facilitating glycolytic reprogramming through the PI3K/AKT signaling pathway.

Indexed as

Cellular ReprogrammingGlycolysisInduced Pluripotent Stem CellsPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSignal TransductionTranscription FactorsAnimalsCells, CulturedChromonesDNA HelicasesMorpholinesNuclear ProteinsSwine2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-oneChromonesDNA HelicasesMorpholinesNuclear ProteinsPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktTranscription FactorsBRG1GlycolysisH3K9me3PI3K/AKT signaling pathwayPorcine iPSCsTranscriptional activity

Identifiers

PMID39643758
PMCPMC11624181

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.