Evidence map›Paper›PMID 39640860›Full record

ArticleFrontiers in microbiology2024

Polymerization potential of a bacterial CotA-laccase for β-naphthol: enzyme structure and comprehensive polymer characterization.

Marina Refaat, Marwa T ElRakaiby, Mustapha El Hariri El Nokab, Julien Es Sayed, Ahmed Elshewy, Khaled O Sebakhy, Nayera Moneib, Tuo Wang, Thomas J Smith, Mohamed H Habib

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In one paragraph

Article in Frontiers in microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Marina RefaatDepartment of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Marwa T ElRakaibyDepartment of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Mustapha El Hariri El NokabDepartment of Chemistry, Michigan State University, East Lansing, MI, United States.
Julien Es SayedZernike Institute for Advanced Materials (ZIAM), University of Groningen, Groningen, Netherlands.
Ahmed ElshewyDepartment of Pharmaceutical Organic Chemistry, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Khaled O SebakhyDepartment of Materials, Textiles and Chemical Engineering, Centre for Polymer and Material Technologies (CPMT), Ghent University, Ghent, Belgium.
Nayera MoneibDepartment of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Tuo WangDepartment of Chemistry, Michigan State University, East Lansing, MI, United States.
Thomas J SmithDepartment of Biochemistry and Molecular Biology, University of Texas Medical Branch (UTMB) at Galveston, Galveston, TX, United States.
Mohamed H HabibDepartment of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

Funding

The role of structural plasticity in the norovirus capsidR01AI141465 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI PETTITT, BERNARD MONTGOMERY, SMITH, THOMAS JAMES. · 2019 to 2022
$2.3M
NIAID NIH HHS R01 AI141465
6 · The paper itself

Abstract

Introduction: Laccases are blue-multicopper containing enzymes that are known to play a role in the bioconversion of recalcitrant compounds. Their role in free radical polymerization of aromatic compounds for their valorization remains underexplored. In this study, we used a pBAD plasmid containing a previously characterized CotA laccase gene (abbreviated as Methods: The protein was expressed from TOP10 cells of Results: The polymerization reaction generated a brown precipitate, and its chemical structure was confirmed using Discussion: This article provides a green/enzymatic pathway for the remediation of phenolics and their valorization into potential useful polymeric materials. The comprehensive analysis of the formed polymer provides insight into its structure and functional moieties present. Based on the yield of the one-gram conversion, this synthetic method proves useful for a pilot-scale production level and opens opportunities to invest in using this polymer for industrial/environmental applications.

Indexed as

crystal structureenzymatic polymerizationexpressionone-gram conversionoptimizationpoly-β-naphthol

Identifiers

PMID39640860
PMCPMC11619140

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.