ArticleData in brief2024
Microscopy and transcriptomic datasets for investigating the drought-stress response and recovery in young and early senescent-old leaves from
Article in Data in brief, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The present dataset combines transcriptomic and microscopic analyses to investigate the responses of winter oilseed rape (WOSR, Brassica napus L., cultivar Aviso) to soil drought, with a focus on differences between young and early-senescent old leaves. For microscopy, 36 scans of 1 to 5 leaf cross-sections were acquired from paraffin-embedded leaf disc samples using a scanner with a 40x lens (Pannoramic Confocal, 3DHistech), capturing a large field of view (8-mm-long observed leaf tissue). The raw scanned cross-sections and analyzed images are available under doi.org/10.57745/RK5PM3 in the Recherche Data Gouvrepository. These high-quality scans enable the differentiation of mesophyll cells and tissues. Software analysis yielded a dataset with 54 selected cross-sectional areas, 291 delimited surfaces of palisade, spongy, and vessel tissues, and 11,136 individually delimited cells from the palisade and spongy layers. For transcriptomics, an Illumina Novaseq sequencer was used to generate 390 Gb of mRNA paired-end reads. The raw reads were filtered, mapped, and assigned to genes from the Brassica napus reference genome
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