ArticleBMC musculoskeletal disorders2024
Integrative bioinformatics analysis reveals novel insights into osteoarthritis pathogenesis and diagnostic biomarkers.
Article in BMC musculoskeletal disorders, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Integrating bioinformatics, molecular dynamics simulation and experimental verification to screen diagnostic biomarkers for polyamine metabolism-related osteoarthritis and predict potential drugs.Journal of orthopaedic surgery and research · 2025Article
- Single-cell sequencing-based study of ferroptosis mechanisms in heat stroke: identification of key biomarkers and dynamic analysis of the immune microenvironment.BMC medical genomics · 2025Article
- Integrating bioinformatics and machine learning to identify biomarkers of branched chain amino acid related genes in osteoarthritis.BMC musculoskeletal disorders · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOsteoarthritis (OA) is a prevalent joint disorder characterized by degeneration and inflammation. Understanding its molecular mechanisms is crucial for diagnosis and treatment.
methodsWe employed bioinformatics analyses to study OA using gene expression data. Differential expression analysis, weighted gene co-expression network analysis (WGCNA), and protein-protein interaction (PPI) network analysis were conducted. Enrichment analyses were performed to elucidate the biological significance of identified genes. Additionally, signature genes were identified using LASSO regression analysis, and a diagnostic nomogram was developed. qRT-PCR was conducted to confirm the expression levels of signature genes.
resultsWe identified 200 differentially expressed genes (DEGs) and a lightgreen module strongly correlated with OA. Within this module, 97 core genes were identified. Fifteen core lipopolysaccharide-related genes (LRGs) were found, enriched in immune and inflammatory pathways. Three hub genes (CCL3, ZFP36, and CCN1) emerged as potential biomarkers for OA diagnosis, with a nomogram showing high predictive accuracy, and validated by using clinical samples. Gene set enrichment analysis (GSEA) revealed distinct signaling pathways associated with the signature genes. Immunological analysis indicated altered immune profiles in OA, with the signature genes influencing immune cell infiltration and immune response pathways.
conclusionOur study provides insights into OA pathogenesis and identifies potential diagnostic biomarkers. The developed nomogram shows promise for accurate OA diagnosis. Furthermore, the signature genes play crucial roles in modulating the immune microenvironment in OA, suggesting their therapeutic potential. CLINICAL TRIAL NUMBER: Not applicable.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.