ArticlePlacenta2025
A transcriptomic comparison of in vitro models of the human placenta.
Article in Placenta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Chemical disruption of placental thyroid hormone signalling: a systematic review that highlights sex-specific effects.Archives of toxicology · 2026Pooled it
- Hippo signaling regulates spontaneous human trophoblast syncytialization in three-dimensional cultures.Molecular human reproduction · 2026Article
- Evidence that S-phase kinase associated protein 2 (SKP2) is ubiquitinated and degraded via a Rho-related BTB domain containing 1 (RhoBTB1) and Cullin-3 mechanism in placenta.Physiological reports · 2026Article
- Characterizing glial cells missing transcription factor 1 (GCM1)'s role in regulating placental gene expression.Placenta · 2026Article
- Natural variation in transplacental transfer efficiency exposes distinct transcriptional network architectures of PFAS effects on birth weight and gestational age.bioRxiv : the preprint server for biology · 2026Article
- SARS-CoV-2 infects human primary cytotrophoblasts mainly through a non-canonical entry route.Molecular human reproduction · 2026Article
- Current approaches and advances in placental toxicology.Trends in endocrinology and metabolism: TEM · 2026Review
- L-(+)-Ergothioneine ameliorates preeclampsia-associated vascular endothelial dysfunction by modulating the Nrf2-PPARγ-sFlt-1 axis.PloS one · 2026Article
- Quercetin protects HTR-8/SVneo human trophoblast cells against oxidative stress injury via activating EGFR/PI3K/AKT-NRF2/HO-1 signaling axis.Frontiers in pharmacology · 2026Article
- Beyond traditional models: microfluidic technologies for engineering the human placentaFrontiers in bioengineering and biotechnology · 2026Review
- Fetal response to maternal exposures of environmental chemicals: Utility of a four-cell human feto-maternal interface organ-on-chip.Chemico-biological interactions · 2025Article
- Effects of selective serotonin reuptake inhibitors on the placenta†.Biology of reproduction · 2025Review
- Investigating the Role of Coenzyme A Restriction in the Pathophysiology of Preeclampsia: Protocol for a Combined Patient Screening and Laboratory Study.JMIR research protocols · 2025Article
- Mechanosensing of Shear Stress and Uterine Spiral Artery Remodeling by Invasive Trophoblasts in Early Pregnancy.International journal of molecular sciences · 2025Review
- PIEZO1 drives trophoblast fusion and placental development.Nature communications · 2025Article
- Associations between maternal plasma concentrations of corticotrophin releasing hormone and the placental transcriptome.Placenta · 2025Article
- Strategy advancements in placental pharmacokinetics: fromFrontiers in pharmacology · 2025Review
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13 authors.
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Abstract
introductionSelecting an in vitro culture model of the human placenta is challenging due to representation of different trophoblast cell types with distinct biological roles and limited comparative studies that define key characteristics of these models. The aim of this research was to compare the transcriptomes of common in vitro models of the human placenta compared to bulk human placental tissue.
methodsWe performed differential gene expression analysis on publicly available transcriptomic data from 7 in vitro models of the human placenta (HTR-8/SVneo, BeWo, JEG-3, JAR, Primary Trophoblasts, Villous Explants, and Trophoblast Stem Cells) and compared to bulk placental tissue from 2 cohort studies (CANDLE and GAPPS) or individual trophoblast cell types derived from bulk placental tissue.
resultsAll in vitro placental models had a substantial number of differentially expressed genes (DEGs, FDR<0.01) compared to the CANDLE and GAPPS placentas (Average DEGs = 10,624), and the individual trophoblast cell types (Average DEGs = 5413), indicating that there are vast differences in gene expression. Hierarchical clustering identified 54 gene clusters with distinct expression profiles across placental models, with 23 clusters enriched for specific KEGG pathways. Placental cell lines were classified by fetal sex based on expression of Y-chromosome genes that identified HTR-8/SVneo cells as female origin, while JEG-3, JAR, and BeWo cells are of male origin. DISCUSSION: None of the models were a close approximation of the human bulk placental transcriptome, highlighting the challenges with model selection. To enable appropriate model selection, we adapted our data into a web application: "Comparative Transcriptomic Placental Model Atlas (CTPMA)".
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