Evidence map›Paper›PMID 39636477›Full record

ArticleJournal of fluorescence2025

A comparative application of spectrophotometric and spectrofluorimetric methods to estimate levofloxacin-DNA and ofloxacin-DNA interactions.

Asiye Üçer, Zehra Ceren Ertekіn, Erdal Dіnç

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Journal of fluorescence, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Asiye ÜçerDepartment of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Yenimahalle, Ankara, Ankara, 06560, Turkey.
Zehra Ceren ErtekіnDepartment of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Yenimahalle, Ankara, Ankara, 06560, Turkey.
Erdal DіnçDepartment of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Yenimahalle, Ankara, Ankara, 06560, Turkey. dinc@ankara.edu.tr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ofloxacin (OFL) and its (S)-enantiomer, levofloxacin (LEV), are among members of the fluoroquinolone antibiotic class, renowned for their broad-spectrum efficacy against both gram-negative and gram-positive bacteria. These potent drugs have been widely used in both human and veterinary medicine, working as bactericidal agents by binding to DNA gyrase, an essential enzyme for bacterial DNA replication. Understanding the binding constants of these drugs to DNA is vital for elucidating their interaction mechanisms and enhancing our grasp of gene expression regulation. The interactions of LEV and OFL with calf thymus DNA under a physiological medium (0.02 M tris-HCl buffer, pH 7.4) using UV spectrophotometry and spectrofluorimetry were investigated. The assay results obtained by applying two spectroscopic approaches confirmed the presence of the interaction of LEV and OFL antibiotics with DNA. In the LEV-DNA and OFL-DNA interactions, hyperchromic effect and fluorescence quenching were observed for UV spectrophotometric and spectrofluorometric measurements, respectively. In the spectrophotometric analysis, the binding constants for the LEV-DNA and OFL-DNA complexes at 298 K were determined as (1.24 ± 0.047) x 10

Indexed as

Anti-Bacterial AgentsDNALevofloxacinOfloxacinAnimalsCattleSpectrometry, FluorescenceSpectrophotometry, UltravioletAnti-Bacterial Agentscalf thymus DNADNALevofloxacinOfloxacinAntibiotic drug-DNA interactionBinding constant determinationLevofloxacinOfloxacinSpectrofluorimetrySpectrophotometry

Identifiers

PMID39636477

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.