ArticleNanophotonics (Berlin, Germany)2022
Four-dimensional multi-particle tracking in living cells based on lifetime imaging.
Article in Nanophotonics (Berlin, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- The Experimental Study of the Validity of the Lagrange-Helmholtz Relationship in Geomagnetic Fields.Sensors (Basel, Switzerland) · 2025Article
- Localizing axial dense emitters based on single-helix point spread function and compressed sensing.Nanophotonics (Berlin, Germany) · 2025Article
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4 authors.
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Abstract
Research on dynamic events in living cells, such as intracellular transportation, is important for understanding cell functions. As movements occur within cells, the microenvironment of the moving vesicles or biomacromolecules may affect the behavior of them. Herein, we propose a method of simultaneously monitoring changes in spatial positions and the local environment related to the fluorescence lifetime, i.e., four-dimensional (4D) multi-particle parallel-tracking in living cells. Based on double-helix point spread function (DH-PSF) microscopy and streak camera, the method combines three-dimensional (3D) localization methods and fluorescence lifetime imaging. By modifying the PSF of the system, the 3D positions and fluorescence lifetime information for several molecules within a depth of a few microns can be acquired simultaneously from a single snapshot. The feasibility of this method is verified by simulating the real-time tracking of a single particle with a given trajectory. In addition, a proof-of-concept 4D tracking system based on the DH-PSF and streak camera was built. The experimental results show that the 3D localization and lifetime precision are
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